Genetic profiling of tumors in PDX models Book Section
| Author: | Poirier, J. T. |
| Editors: | Uthamanthil, R.; Tinkey, P.; de Stanchina, E. |
| Article/Chapter Title: | Genetic profiling of tumors in PDX models |
| Abstract: | Patient-derived xenografts (PDXs) are useful for therapeutic analysis in mice. However, the power of these models is substantially increased by detailed characterization. The genomic characterization of PDX models presents a unique set of challenges for laboratory researchers due to the presence of murine cells around and within the tumor. Standard approaches to next-generation sequencing optimized for human tissues may fail to yield useful results if these challenges are not taken into consideration. Mutational analysis is particularly susceptible to false-positives resulting from contaminating mouse sequences misaligned to the human reference genome. Quantitation of the presence and degree of mouse contamination of a DNA sample is recommended to avoid attempts to sequence samples with a high degree of murine contamination. Selective depletion of murine stroma and bioinformatic pipelines designed for dealing with admixed DNA are a requirement for accurate characterization of tumor xenografts grown in mice. © 2017 Elsevier Inc. All rights reserved. |
| Keywords: | xenograft; stroma; sequencing; characterization; alignment; hybrid; profiling; mapping; sspal |
| Book Title: | Patient Derived Tumor Xenograft Models: Promise, Potential and Practice |
| ISBN: | 978-0-12-804010-2 |
| Publisher: | Academic Press, Elsevier Inc |
| Publication Place: | London, UK |
| Date Published: | 2017-01-01 |
| Start Page: | 149 |
| End Page: | 159 |
| Language: | English |
| DOI: | 10.1016/b978-0-12-804010-2.00011-4 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Section II Chapter 5 -- Export Date: 3 July 2017 -- Source: Scopus |
