Diversity of the Tcra-V3 gene family in BALB/c mice Journal Article
| Authors: | Tan, K. N.; Huang, K.; Myrick, K. V.; Tanigawa, C. |
| Article Title: | Diversity of the Tcra-V3 gene family in BALB/c mice |
| Abstract: | Southern analysis of Eco RI-digested BALB/c liver DNA reveals four T-cell receptor Tcra-V3-hybridizing DNA fragments, which are of sizes 18.0, 12.0, 8.0, and 2.1 kilobases, respectively. These four Tcra-V3-hybridizing genomic DNA were isolated from a BALB/c genomic library. Restriction and Southern analysis of the genomic DNA clones showed that each of the Tcra-V3-hybridizing Eco RI DNA fragments harbors only a single Tcra-V3 gene. The DNA sequences of coding regions of the four Tcra-V3 family members were determined. These sequences show very limited divergence from one another. Comparisons of BALB/c Tcra-V3 sequences with published Tcra-V3 sequences expressed in different strains of mice reveal substantial allelic polymorphism. Sequence similarity searches retrieved homologous rat, cattle, and human genes. The scarcity of coding sequence divergence among members of the Tcra-V3 family and the more substantial allelic polymorphism may be general features of the T-cell receptor V-alpha chain-encoding gene families. |
| Keywords: | nonhuman; mouse; animals; mice; genetic variability; gene library; mice, inbred balb c; mice, inbred c57bl; cloning, molecular; t lymphocyte receptor; liver; amino acid sequence; molecular sequence data; sequence homology, amino acid; recombinant fusion proteins; sequence alignment; base sequence; dna sequence; receptors, antigen, t-cell, alpha-beta; multigene family; dna, complementary; variation (genetics); t lymphocyte receptor gene; priority journal; article |
| Journal Title: | Immunogenetics |
| Volume: | 44 |
| Issue: | 5 |
| ISSN: | 0093-7711 |
| Publisher: | Springer |
| Date Published: | 1996-09-01 |
| Start Page: | 372 |
| End Page: | 376 |
| Language: | English |
| DOI: | 10.1007/s002510050139 |
| PUBMED: | 8781123 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work