Assignment of the gene for the β subunit of thyroid-stimulating hormone to the short arm of human chromosome 1 Journal Article
| Authors: | Dracopoli, N. C.; Rettig, W. J.; Whitfield, G. K.; Darlington, G. J.; Spengler, B. A.; Biedler, J. L.; Old, L. J.; Kourides, I. A. |
| Article Title: | Assignment of the gene for the β subunit of thyroid-stimulating hormone to the short arm of human chromosome 1 |
| Abstract: | The chromosomal locations of the genes for the β subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone α subunit have been determined by restriction enzyme analysis of DNA extracted from rodent-human somatic cell hybrids. Human chorionic gonadotropin (CG) α-subunit cDNA and a cloned 0.9-kilobase (kb) fragment of the human TSH β-subunit gene were used as hybridization probes in the analysis of Southern blots of DNA extracted from rodent-human hybrid clones. Analysis of the segregation of 5- and 10-kb EcoRI fragments hybridizing to CG α-subunit cDNA confirmed the previous assignment of this gene to chromosome 6. Analysis of the patterns of segregation of a 2.3-kb EcoRI fragment containing human TSH β-subunit sequences permitted the assignment of the TSH β-subunit gene to human chromosome 1. The subregional assignment of TSH β subunit to chromosome 1p22 was made possible by the additional analysis of a set of hybrids containing partially overlapping segments of this chromosome. Human TSH β subunit is not syntenic with genes encoding the β subunits of CG, luteinizing hormone, or follicle-stimulating hormone and is assigned to a conserved linkage group that also contains the structural genes for the β subunit of nerve growth factor (NGFB) and the proto-oncogene N-ras (NRAS). |
| Keywords: | unclassified drug; human cell; heredity; gene mapping; genetic engineering; rodentia; gene location; chromosome 1p; thyrotropin; endocrine system; normal value; human; priority journal; article; gene assignment; glycoprotein hormone |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 83 |
| Issue: | 6 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1986-03-01 |
| Start Page: | 1822 |
| End Page: | 1826 |
| Language: | English |
| DOI: | 10.1073/pnas.83.6.1822 |
| PROVIDER: | scopus |
| PMCID: | PMC323176 |
| PUBMED: | 3006075 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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