New imaging probes to track cell fate: Reporter genes in stem cell research Journal Article
| Authors: | Jurgielewicz, P.; Harmsen, S.; Wei, E.; Bachmann, M. H.; Ting, R.; Aras, O. |
| Article Title: | New imaging probes to track cell fate: Reporter genes in stem cell research |
| Abstract: | Cell fate is a concept used to describe the differentiation and development of a cell in its organismal context over time. It is important in the field of regenerative medicine, where stem cell therapy holds much promise but is limited by our ability to assess its efficacy, which is mainly due to the inability to monitor what happens to the cells upon engraftment to the damaged tissue. Currently, several imaging modalities can be used to track cells in the clinical setting; however, they do not satisfy many of the criteria necessary to accurately assess several aspects of cell fate. In recent years, reporter genes have become a popular option for tracking transplanted cells, via various imaging modalities in small mammalian animal models. This review article examines the reporter gene strategies used in imaging modalities such as MRI, SPECT/PET, Optoacoustic and Bioluminescence Imaging. Strengths and limitations of the use of reporter genes in each modality are discussed. © 2017, Springer International Publishing AG. |
| Keywords: | genetics; nonhuman; nuclear magnetic resonance imaging; animal; animals; animal model; cell fate; stem cell transplantation; cell differentiation; pathology; molecular imaging; diagnostic imaging; stem cell; disease model; engraftment; mammal; reporter gene; stem cells; cell therapy; genes, reporter; bioluminescence; cell tracking; comparative effectiveness; single photon emission computed tomography; regenerative medicine; stem cell research; procedures; humans; human; organ culture; dna probe; cell imaging modalities; stem cell engraftment; stem cell tracking |
| Journal Title: | Cellular and Molecular Life Sciences |
| Volume: | 74 |
| Issue: | 24 |
| ISSN: | 1420-682X |
| Publisher: | Springer |
| Date Published: | 2017-12-01 |
| Start Page: | 4455 |
| End Page: | 4469 |
| Language: | English |
| DOI: | 10.1007/s00018-017-2584-z |
| PUBMED: | 28674728 |
| PROVIDER: | scopus |
| PMCID: | PMC5665705 |
| DOI/URL: | |
| Notes: | Review -- Export Date: 1 December 2017 -- Source: Scopus |
