Imaging T cell dynamics and function using PET and human nuclear reporter genes Journal Article
| Authors: | Lee, J. T.; Moroz, M. A.; Ponomarev, V. |
| Article Title: | Imaging T cell dynamics and function using PET and human nuclear reporter genes |
| Abstract: | Adoptive cell transfer immunotherapy has demonstrated significant promise in the treatment of cancer, with long-term, durable responses. T cells expressing T cell receptors (TCRs) that recognize tumor antigens, or engineered with chimeric antigen receptors (CARs) can recognize and eliminate tumor cells even in advanced disease. Positron emission tomography (PET) imaging with nuclear reporter genes, a noninvasive method to track and monitor function of engineered cells in vivo, allows quantitative, longitudinal monitoring of these cells, including their expansion/contraction, migration, retention at target and off-target sites, and biological state. As an additional advantage, some reporter genes also exhibit “suicide potential” permitting the safe elimination of adoptively transferred T cells in instances of adverse reaction to therapy. Here, we describe the production of human nuclear reporter gene-expressing T cells and noninvasive PET imaging to monitor their cell fate in vivo. © Springer Science+Business Media, LLC, part of Springer Nature 2018. |
| Keywords: | immunotherapy; pet; t cell; chimeric antigen receptors; reporter probe; human nuclear reporter gene; nucleoside kinases |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1790 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2018-01-01 |
| Start Page: | 165 |
| End Page: | 180 |
| Language: | English |
| DOI: | 10.1007/978-1-4939-7860-1_13 |
| PROVIDER: | scopus |
| PUBMED: | 29858791 |
| PMCID: | PMC9344925 |
| DOI/URL: | |
| Notes: | Chapter 13 in "Reporter Gene Imaging: Methods and Protocols" (ISBN: 978-1-4939-7858-8) -- Export Date: 2 July 2018 -- Source: Scopus |
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