RNA helicase A mediates association of CBP with RNA polymerase II Journal Article
| Authors: | Nakajima, T.; Uchida, C.; Anderson, S. F.; Lee, C. G.; Hurwitz, J.; Parvin, J. D.; Montminy, M. |
| Article Title: | RNA helicase A mediates association of CBP with RNA polymerase II |
| Abstract: | The coactivator CBP has been proposed to stimulate the expression of certain signal-dependent genes via its association with RNA polymerase II complexes. Here we show that complex formation between CBP and RNA polymerase II requires RNA helicase A (RHA), a nuclear DNA/RNA helicase that is related to the Drosophila male dosage compensation factor role. In transient transfection assays, RHA was found to cooperate with CBP in mediating target gene activation via the CAMP responsive factor CREB. As a mutation in RHA that compromised its helicase activity correspondingly reduced CREB-dependent transcription, we propose that RHA may induce local changes in chromatin structure that promote engagement of the transcriptional apparatus on signal responsive promoters. |
| Keywords: | signal transduction; nonhuman; animals; cells, cultured; complex formation; protein binding; drosophila; transcription, genetic; enzyme activity; gene induction; rna helicase; rna helicases; rna polymerase ii; phosphoserine; chromatin structure; dna, complementary; cyclic amp response element-binding protein; rna polymerase; gene expression regulation, enzymologic; zinc fingers; rna nucleotidyltransferases; precipitin tests; humans; human; male; priority journal; article |
| Journal Title: | Cell |
| Volume: | 90 |
| Issue: | 6 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1997-09-19 |
| Start Page: | 1107 |
| End Page: | 1112 |
| Language: | English |
| DOI: | 10.1016/s0092-8674(00)80376-1 |
| PUBMED: | 9323138 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
