An ATPase component of the transcription elongation complex is required for factor-dependent transcription termination by vaccinia RNA polymerase Journal Article

  


Authors: Deng, L.; Shuman, S.
Article Title: An ATPase component of the transcription elongation complex is required for factor-dependent transcription termination by vaccinia RNA polymerase
Abstract: Vaccinia virus RNA polymerase terminates transcription in response to a specific signal UUUUUNU in the nascent transcript. Transduction of this signal to the elongating polymerase requires a virus-encoded termination factor, VTF. The existence of a second termination factor was suggested by the finding that transient exposure of purified elongation complexes to heparin rendered them refractory to VTF-induced termination. Loss of termination competence correlated with the removal of several polypeptide components of the elongation complex. We present the identification of factor X, an activity that restored VTF responsiveness to heparin-stripped ternary complexes. We propose that factor X, which has an associated DNA-dependent ATPase activity, mediates the requirement for ATP hydrolysis during transcription termination.
Keywords: nonhuman; transcription, genetic; genetic transduction; molecular sequence data; nucleotide sequence; vaccinia virus; heparin; base sequence; adenosine triphosphatase; adenosine triphosphatases; hydrolysis; dna-directed rna polymerases; rna polymerase; elongation factor; vaccinia; transcription termination; dna template; dna protein complex; dna, recombinant; priority journal; article; blood clotting factor 10; terminator regions (genetics)
Journal Title: Journal of Biological Chemistry
Volume: 271
Issue: 46
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1996-11-15
Start Page: 29386
End Page: 29392
Language: English
DOI: 10.1074/jbc.271.46.29386
PUBMED: 8910603
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029825580&doi=10.1074%2fjbc.271.46.29386&partnerID=40&md5=1eb3ddf8ec1814fe46758d3ca5cea7a1
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
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MSK Authors
  1. Liang Deng
    83 Deng
  2. Stewart H Shuman
    546 Shuman
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