Cdk7 is required for full activation of Drosophila heat shock genes and RNA polymerase II phosphorylation in vivo Journal Article

Authors: Schwartz, B. E.; Larochelle, S.; Suter, B.; Lis, J. T.
Article Title: Cdk7 is required for full activation of Drosophila heat shock genes and RNA polymerase II phosphorylation in vivo
Abstract: TFIIH has been implicated in several fundamental cellular processes, including DNA repair, cell cycle progression, and transcription. In transcription, the helicase activity of TFIIH functions to melt promoter DNA; however, the in vivo function of the Cdk7 kinase subunit of TFIIH, which has been hypothesized to be involved in RNA polymerase II (Pol II) phosphorylation, is not clearly understood. Using temperature-sensitive and null alleles of cdk7, we have examined the role of Cdk7 in the activation of Drosophila heat shock genes. Several in vivo approaches, including polytene chromosome immunofluorescence, nuclear run-on assays, and, in particular, a protein-DNA cross-linking assay customized for adults, revealed that Cdk7 kinase activity is required for full activation of heat shock genes, promoter-proximal Pol II pausing, and Pol II-dependent chromatin decondensation. The requirement for Cdk7 occurs very early in the transcription cycle. Furthermore, we provide evidence that TFIIH associates with the elongation complex much longer than previously suspected.
Keywords: promoter region; nonhuman; animals; allele; drosophila; transcription, genetic; enzyme activation; immunofluorescence; enzyme activity; phosphorylation; transcription factors; gene activation; enzyme phosphorylation; rna, messenger; chromatin; recombinant proteins; temperature; helicase; protein subunits; heat-shock response; point mutation; cyclin-dependent kinases; drosophila proteins; rna polymerase ii; temperature sensitivity; cyclin dependent kinase 7; hexapoda; gene expression regulation, enzymologic; cross linking; animals, genetically modified; chromatin condensation; dna protein complex; heat-shock proteins; heat shock protein; promoter regions (genetics); chicken ovalbumin upstream promoter transcription factor; priority journal; article; gallus gallus; arachnida; polytene chromosome
Journal Title: Molecular and Cellular Biology
Volume: 23
Issue: 19
ISSN: 0270-7306
Publisher: American Society for Microbiology  
Date Published: 2003-10-01
Start Page: 6876
End Page: 6886
Language: English
DOI: 10.1128/mcb.23.19.6876-6886.2003
PUBMED: 12972606
PROVIDER: scopus
PMCID: PMC193928
Notes: Export Date: 12 September 2014 -- Source: Scopus
Citation Impact
MSK Authors