Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH Journal Article
| Authors: | Reardon, J. T.; Ge, H.; Gibbs, E.; Sancar, A.; Hurwitz, J.; Pan, Z. Q. |
| Article Title: | Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH |
| Abstract: | Transcription factor IIH (TFIIH) is a multisubunit protein complex essential for both the initiation of RNA polymerase class II (pol II)- catalyzed transcription and nucleotide excision repair of DNA. Recent studies have shown that TFIIH copurifies with the cyclin-dependent kinase (cdk)activating kinase complex (CAK) that includes cdk7, cyclin H, and p36/MAT1. Here we report the isolation of two TFIIH-related complexes: TFIIH* and ERCC2/CAK. TFIIH* consists of a subset of the TFIIH complex proteins including ERCC3 (XPB), p62, p44, p41, and p34 but is devoid of detectable levels of ERCC2 (XPD) and CAK. ERCC2/CAK was isolated as a complex that exhibits CAK activity that cosediments with the three CAK components (cdk7, cyclin H, and p36/MAT1) as well as the ERCC2 (XPD) protein. TFIIH* can support pol II-catalyzed transcription in vitro with lower efficiency compared with TFIIH. This TFIIH*-dependent transcription reaction was stimulated by ERCC2/CAK. The ERCC2/CAK and TFIIH* complexes are each active in DNA repair as shown by their ability to complement extracts prepared from ERCC2 (XPD)- and ERCC3 (XPB)-deficient cells, respectively, in supporting the excision of DNA containing a cholesterol lesion. These data suggest that TFIIH* and ERCC2/CAK interact to form the TFIIH holoenzyme capable of efficiently assembling the pol II transcription initiation complex and directly participating in excision repair reactions. |
| Keywords: | controlled study; dna-binding proteins; nonhuman; proteins; animal cell; animals; dna repair; transcription initiation; molecular dynamics; transcription factor; transcription, genetic; transcription factors; transcription regulation; transcription; excision repair; cyclin-dependent kinases; cyclin dependent kinase; cell cycle regulation; protein determination; rna polymerase ii; dna helicases; protein isolation; transcription factor tfiih; cho cell; cho cells; cricetinae; chromatography, ion exchange; xeroderma pigmentosum group d protein; transcription factors, tfii; humans; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 93 |
| Issue: | 13 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1996-06-25 |
| Start Page: | 6482 |
| End Page: | 6487 |
| Language: | English |
| DOI: | 10.1073/pnas.93.13.6482 |
| PUBMED: | 8692841 |
| PROVIDER: | scopus |
| PMCID: | PMC39049 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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