The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro Journal Article
| Authors: | Lu, H.; Fisher, R. P.; Bailey, P.; Levine, A. J. |
| Article Title: | The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro |
| Abstract: | Phosphorylation is believed to be one of the mechanisms by which p53 becomes activated or stabilized in response to cellular stress. Previously, p53 was shown to interact with three components of transcription factor IIH (TFIIH): excision repair cross-complementing types 2 and 3 (ERCC2 and ERCC3) and p62. This communication demonstrates that p53 is phosphorylated by the TFIIH-associated kinase in vitro. The phosphorylation was found to be catalyzed by the highly purified kinase components of TFIIH, the CDK7-cycH- p36 trimeric complex. The phosphorylation sites were mapped to the C-terminal amino acids located between residues 311 and 393. Serines 371, 376, 378, and 392 may be the potential sites for this kinase. Phosphorylation of p53 by this kinase complex enhanced the ability of p53 to bind to the sequence- specific p53-responsive DNA element as shown by gel mobility shift assays. These results suggest that the CDK7-cycH-p36 trimeric complex of TFIIH may play a role in regulating p53 functions in cells. |
| Keywords: | protein phosphorylation; mutation; binding affinity; protein conformation; metabolism; cell function; serine; carboxy terminal sequence; protein binding; transcription factor; enzyme activity; hela cell; hela cells; protein serine threonine kinase; phosphorylation; protein p53; transcription factors; chemistry; dna; hybrid protein; recombinant fusion proteins; protein-serine-threonine kinases; tumor suppressor protein p53; dna sequence; excision repair; catalysis; dna binding; cyclin-dependent kinases; cyclin dependent kinase; isolation and purification; cyclin dependent kinase activating kinase; cyclin-dependent kinase-activating kinase; transcription factor iih; transcription factor tfiih; transcription factors, tfii; humans; human; priority journal; article; transcription factor ii |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 17 |
| Issue: | 10 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1997-10-01 |
| Start Page: | 5923 |
| End Page: | 5934 |
| Language: | English |
| PUBMED: | 9315650 |
| PROVIDER: | scopus |
| DOI: | 10.1128/MCB.17.10.5923 |
| PMCID: | PMC232440 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
