Inhibition of transcription by the trimeric cyclin-dependent kinase 7 complex Journal Article
| Authors: | Bochar, D. A.; Pan, Z. Q.; Knights, R.; Fisher, R. P.; Shilatifard, A.; Shiekhattar, R. |
| Article Title: | Inhibition of transcription by the trimeric cyclin-dependent kinase 7 complex |
| Abstract: | Cyclin-dependent kinase 7 (CDK7) can be isolated as a subunit of a trimeric kinase complex functional in activation of the mitotic promoting factor. In this study, we demonstrate that the trimeric cdk-activating kinase (CAK) acts as a transcriptional repressor of class II promoters and show that repression results from CAK impeding the entry of RNA polymerase II and basal transcription factor IIF into a competent preinitiation complex. This repression is independent of CDK7 kinase activity. We find that the p36/MAT1 subunit of CAK is required for transcriptional repression and the repression is independent of the promoter used. Our results demonstrate a central role for CAK in regulation of messenger RNA synthesis by either inhibition of RNA polymerase II-catalyzed transcription or stimulation of transcription through association with basal transcription repair factor IIH. |
| Keywords: | human cell; promoter region; complex formation; enzyme activity; molecular cloning; transcription regulation; excision repair; drosophila melanogaster; catalysis; sequence homology; cyclin dependent kinase; enzyme subunit; enzyme isolation; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 274 |
| Issue: | 19 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1999-05-07 |
| Start Page: | 13162 |
| End Page: | 13166 |
| Language: | English |
| DOI: | 10.1074/jbc.274.19.13162 |
| PUBMED: | 10224071 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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