RNA helicase A is essential for normal gastrulation Journal Article

   


Authors: Lee, C. G.; Da Costa Soares, V.; Newberger, C.; Manova, K.; Lacy, E.; Hurwitz, J.
Article Title: RNA helicase A is essential for normal gastrulation
Abstract: RNA helicase A (RHA) is the human homologue of the Drosophila maleless protein, an essential factor for the development of male flies. Recently, it was shown that RHA cooperates with the cAMP-responsive element in mediating the cAMP-dependent transcriptional activation of a number of genes. Due to the participation of cAMP as a second messenger in a number of signaling pathways, we examined the function of RHA during mammalian embryogenesis. To examine the role(s) of RHA in mammalian development, RHA knockout mice were generated by homologous recombination. Homozygosity for the mutant RHA allele led to early embryonic lethality. Histological analysis, combined with terminal deoxynucleotidyltransferase-mediated UTP end labeling (TUNEL) reactions of RHA-null embryos, revealed marked apoptotic cell death specifically in embryonic ectodermal cells during gastrulation. RNA in situ analyses of the expression of HNF-3β and Brachyury, two molecular markers for gastrulation, showed that RHA-null embryos at days 7.5 and 8.5 expressed both HNF-3β and Brachyury in a pattern similar to those of pre- and early streak stages of embryos, respectively. These observations indicate that RHA is necessary for early embryonic development and suggest the requirement of RHA for the survival and differentiation of embryonic ectoderm.
Keywords: controlled study; mutation; nonhuman; animal cell; mouse; animals; mice; mice, knockout; animal tissue; cell survival; embryo; animal experiment; genotype; genetic transcription; cell differentiation; histology; stem cell; in situ hybridization; gene expression regulation, developmental; homozygosity; dna; recombination, genetic; rna helicase; rna helicases; cyclic amp responsive element binding protein; gastrulation; southern blotting; ectoderm; gastrula; humans; male; priority journal; article; embryonic and fetal development; cyclic amp responsive element
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 95
Issue: 23
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1998-11-10
Start Page: 13709
End Page: 13713
Language: English
DOI: 10.1073/pnas.95.23.13709
PUBMED: 9811865
PROVIDER: scopus
PMCID: PMC24884
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032506230&doi=10.1073%2fpnas.95.23.13709&partnerID=40&md5=f8d8e1df44e926a2da51701e6bf976e8
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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MSK Authors
  1. Jerard Hurwitz
    206 Hurwitz
  2. Elizabeth H Lacy
    74 Lacy
  3. Katia O Manova-Todorova
    161 Manova-Todorova
  4. Chee-Gun Lee
    11 Lee
  5. Vera Soares
    22 Soares
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