Molecular analysis of the cDNA and genomic DNA encoding mouse RNA helicase A Journal Article
| Authors: | Lee, C. G.; Eki, T.; Okumura, K.; da Costa Soares, V.; Hurwitz, J. |
| Article Title: | Molecular analysis of the cDNA and genomic DNA encoding mouse RNA helicase A |
| Abstract: | RNA helicase A is an enzyme that possesses both RNA and DNA helicase activities. In this report, we describe the isolation of a mouse cDNA encoding RNA helicase A. The deduced amino acid sequence derived from mouse RNA helicase A cDNA exhibits 87 and 47% identity to its human and Drosophila homologs, respectively. Using Southern blot analysis employing a mouse backcross panel, we have assigned the mouse RNA helicase A gene to chromosome 1, mapping near the D1Bir20 locus at MGD position 67. Northern blot and primer extension analyses indicate that, although its level is variable, RNA helicase A appears to be expressed from a single transcription start site in all tissues tested. Sequence analysis of the upstream genomic DNA revealed that the promoter region lacks a TATA box and contains two high affinity sites for Sp1, one ISRE, a binding site for interferon regulatory factor, and three AP2-binding sites. These findings suggest that the transcriptional regulation of the RNA helicase A gene is complex. |
| Keywords: | unclassified drug; sequence analysis; exons; nonhuman; molecular genetics; binding affinity; animal cell; chromosome 1; mouse; animals; mice; embryo; introns; mice, inbred c57bl; dna; transcription regulation; amino acid sequence; molecular sequence data; enzyme analysis; rna, messenger; nucleotide sequence; organ specificity; transcription factor sp1; base sequence; binding site; sequence homology; rna helicase; rna helicase a; rna helicases; dna determination; complementary dna; chromosome mapping; dna, complementary; sequence analysis, dna; regulatory sequences, nucleic acid; tata box; transcription factor ap 2; rna nucleotidyltransferases; priority journal; article |
| Journal Title: | Genomics |
| Volume: | 47 |
| Issue: | 3 |
| ISSN: | 0888-7543 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1998-02-01 |
| Start Page: | 365 |
| End Page: | 371 |
| Language: | English |
| DOI: | 10.1006/geno.1997.5139 |
| PUBMED: | 9480750 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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