Molecular analysis of the cDNA and genomic DNA encoding mouse RNA helicase A Journal Article


Authors: Lee, C. G.; Eki, T.; Okumura, K.; da Costa Soares, V.; Hurwitz, J.
Article Title: Molecular analysis of the cDNA and genomic DNA encoding mouse RNA helicase A
Abstract: RNA helicase A is an enzyme that possesses both RNA and DNA helicase activities. In this report, we describe the isolation of a mouse cDNA encoding RNA helicase A. The deduced amino acid sequence derived from mouse RNA helicase A cDNA exhibits 87 and 47% identity to its human and Drosophila homologs, respectively. Using Southern blot analysis employing a mouse backcross panel, we have assigned the mouse RNA helicase A gene to chromosome 1, mapping near the D1Bir20 locus at MGD position 67. Northern blot and primer extension analyses indicate that, although its level is variable, RNA helicase A appears to be expressed from a single transcription start site in all tissues tested. Sequence analysis of the upstream genomic DNA revealed that the promoter region lacks a TATA box and contains two high affinity sites for Sp1, one ISRE, a binding site for interferon regulatory factor, and three AP2-binding sites. These findings suggest that the transcriptional regulation of the RNA helicase A gene is complex.
Keywords: unclassified drug; sequence analysis; exons; nonhuman; molecular genetics; binding affinity; animal cell; chromosome 1; mouse; animals; mice; embryo; introns; mice, inbred c57bl; dna; transcription regulation; amino acid sequence; molecular sequence data; enzyme analysis; rna, messenger; nucleotide sequence; organ specificity; transcription factor sp1; base sequence; binding site; sequence homology; rna helicase; rna helicase a; rna helicases; dna determination; complementary dna; chromosome mapping; dna, complementary; sequence analysis, dna; regulatory sequences, nucleic acid; tata box; transcription factor ap 2; rna nucleotidyltransferases; priority journal; article
Journal Title: Genomics
Volume: 47
Issue: 3
ISSN: 0888-7543
Publisher: Elsevier Inc.  
Date Published: 1998-02-01
Start Page: 365
End Page: 371
Language: English
DOI: 10.1006/geno.1997.5139
PUBMED: 9480750
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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  1. Jerard Hurwitz
    206 Hurwitz
  2. Chee-Gun   Lee
    11 Lee