A cDNA clone for a novel nuclear protein with DNA binding activity Journal Article
| Authors: | Mattioni, T.; Hume, C. R.; Konigorski, S.; Hayes, P.; Osterweil, Z.; Lee, J. S. |
| Article Title: | A cDNA clone for a novel nuclear protein with DNA binding activity |
| Abstract: | In an effort to identify trans-acting factors regulating specific genes, we cloned a novel human gene, DBP-5. The cDNA clone contains a predicted open reading frame coding for a potential 1,179 amino acid protein. The mRNA corresponding to DBP-5 is ubiquitously distributed, and the gene is phylogenetically conserved. Immunofluorescence analyses with several cell lines indicate that the protein is localized to the nucleus. Sequence analysis revealed unusual features of the predicted protein structure, including four completely conserved repeats. The phylogenetic conservation of DBP-5, the ubiquity of its expression, its nuclear localization, and its ability to bind DNA sequences, raise the possibility that DBP-5 may play a role in the organization of interphase chromatin and/or in transcriptional regulation. © 1992 Springer-Verlag. |
| Keywords: | dna binding protein; dna-binding proteins; gene expression; immunofluorescence; hela cells; nuclear proteins; cloning, molecular; dna; amino acid sequence; molecular sequence data; recombinant fusion proteins; tissue distribution; nucleotide sequence; base sequence; antibody specificity; cell nucleus; dna binding; repetitive sequences, nucleic acid; dna probes; human; priority journal; article; trans acting factor; support, u.s. gov't, p.h.s. |
| Journal Title: | Chromosoma |
| Volume: | 101 |
| Issue: | 10 |
| ISSN: | 0009-5915 |
| Publisher: | Springer |
| Date Published: | 1992-10-01 |
| Start Page: | 618 |
| End Page: | 624 |
| Language: | English |
| DOI: | 10.1007/bf00360539 |
| PUBMED: | 1424986 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
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