Imaging approaches to investigate myonuclear positioning in drosophila Journal Article


Authors: Azevedo, M.; Schulman, V. K.; Folker, E.; Balakrishnan, M.; Baylies, M.
Article Title: Imaging approaches to investigate myonuclear positioning in drosophila
Title Series: Methods in Molecular Biology
Abstract: In the skeletal muscle, nuclei are positioned at the periphery of each myofiber and are evenly distributed along its length. Improper positioning of myonuclei has been correlated with muscle disease and decreased muscle function. Several mechanisms required for regulating nuclear position have been identified using the fruit fly, Drosophila melanogaster. The conservation of the myofiber between the fly and vertebrates, the availability of advanced genetic tools, and the ability to visualize dynamic processes using fluorescent proteins in vivo makes the fly an excellent system to study myonuclear positioning. This chapter describes time-lapse and fixed imaging methodologies using both the Drosophila embryo and the larva to investigate mechanisms of myonuclear positioning.
Keywords: embryo; protein; drosophila; morphogenesis; myoblast fusion; muscle; expression; specification; cell fates; melanogaster; integration; nuclear movement; somatic musculature; larvae
Journal Title: Methods in Molecular Biology
Volume: 1411
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2016-01-01
Start Page: 291
End Page: 312
Language: English
ACCESSION: WOS:000381767700020
PROVIDER: wos
PMCID: PMC4939885
PUBMED: 27147050
DOI: 10.1007/978-1-4939-3530-7_19
Notes: Chapter 19 in "The Nuclear Envelope" (ISBN: 978-1-4939-3528-4) -- Source: Wos
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MSK Authors
  1. Eric Folker
    8 Folker
  2. Mary K Baylies
    71 Baylies