A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme Journal Article
| Authors: | Wittschieben, B. Ø; Otero, G.; De Bizemont, T.; Fellows, J.; Erdjument-Bromage, H.; Ohba, R.; Li, Y.; Allis, C. D.; Tempst, P.; Svejstrup, J. Q. |
| Article Title: | A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme |
| Abstract: | The elongator complex is a major component of the RNA polymerase II (RNAPII) holoenzyme responsible for transcriptional elongation in yeast. Here we identify Elp3, the 60-kilodalton subunit of elongator/RNAPII holoenzyme, as a highly conserved histone acetyltransferase (HAT) capable of acetylating core histones in vitro. In vivo, ELP3 gene deletion confers typical elp phenotypes such as slow growth adaptation, slow gene activation, and temperature sensitivity. These results suggest a rote for a novel, tightly RNAPII-associated HAT in transcription of DNA packaged in chromatin. |
| Keywords: | gene deletion; nonhuman; phenotype; cloning, molecular; gene activation; amino acid sequence; molecular sequence data; enzyme analysis; saccharomyces cerevisiae; sequence alignment; chromatin; recombinant proteins; yeast; saccharomyces cerevisiae proteins; enzyme structure; rna polymerase ii; temperature sensitivity; acetyltransferases; fungal proteins; histone acetyltransferase; histone acetyltransferases; article |
| Journal Title: | Molecular Cell |
| Volume: | 4 |
| Issue: | 1 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 1999-07-01 |
| Start Page: | 123 |
| End Page: | 128 |
| Language: | English |
| DOI: | 10.1016/s1097-2765(00)80194-x |
| PUBMED: | 10445034 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
