Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation Journal Article
| Authors: | Otero, G.; Fellows, J.; Yang, L.; De Bizemont, T.; Dirac, A. M. G.; Gustafsson, C. M.; Erdjument-Bromage, H.; Tempst, P.; Svejstrup, J. Q. |
| Article Title: | Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation |
| Abstract: | The form of RNA polymerase II (RNAPII) engaged in transcriptional elongation was isolated. Elongating RNAPII was associated with a novel multisubunit complex, termed elongator, whose stable interaction was dependent on a hyperphosphorylated state of the RNAPII carboxy-terminal domain (CTD). A free form of elongator was also isolated, demonstrating the discrete nature of the complex, and free elongator could bind directly to RNAPII. The gene encoding the largest subunit of elongator, ELP1, was cloned. Phenotypes of yeast elp1Δ, cells demonstrated an involvement of elongator in transcriptional elongation as well as activation in vivo. Our data indicate that the transition from transcriptional initiation to elongation involves an exchange of the multiprotein mediator complex for elongator in a reaction coupled to CTD hyperphosphorylation. |
| Keywords: | protein expression; protein phosphorylation; nonhuman; protein analysis; phenotype; cell division; protein protein interaction; protein binding; transcription, genetic; phosphorylation; transcription factors; gene expression regulation; cloning, molecular; gene activation; transcription regulation; saccharomyces cerevisiae; rna translation; rna polymerase ii; transcriptional elongation factors; elongation factor; fungal proteins; trans-activation (genetics); article; transcription factors, general |
| Journal Title: | Molecular Cell |
| Volume: | 3 |
| Issue: | 1 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 1999-01-01 |
| Start Page: | 109 |
| End Page: | 118 |
| Language: | English |
| DOI: | 10.1016/s1097-2765(00)80179-3 |
| PUBMED: | 10024884 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
