Cloning of novel injury-regulated genes: Implications for an important role of the muscle-specific protein skNAC in muscle repair Journal Article
| Authors: | Munz, B.; Wiedmann, M.; Lochmüller, H.; Werner, S. |
| Article Title: | Cloning of novel injury-regulated genes: Implications for an important role of the muscle-specific protein skNAC in muscle repair |
| Abstract: | To gain insight into the molecular mechanisms underlying the wound repair process, we searched for genes that are regulated by skin injury. Using the differential display reverse transcription-polymerase chain reaction technique, we identified a gene that was strongly induced as early as 12 h after wounding. Sequence analysis revealed the identity of the corresponding protein with skeletal muscle nascent polypeptide-associated complex (skNAC), a recently identified muscle-specific transcription factor. By in situ hybridization and immunohistochemistry, we demonstrated the specific expression of skNAC in skeletal muscle cells of the panniculus carnosus at the wound edge. Furthermore, in vitro studies with cultured myoblasts revealed expression of skNAC in differentiating and differentiated, but not in proliferating, nondifferentiated cells. Differentiation of cultured myoblasts was accompanied by simultaneous expression of skNAC and the muscle-specific transcription factor myogenin. Our results provide the first evidence for a role of skNAC in muscle repair processes. Furthermore, they demonstrate the usefulness of our approach in identifying new players in wound repair. |
| Keywords: | protein expression; nonhuman; mouse; animals; mice; reverse transcription polymerase chain reaction; cell line; animal experiment; cell differentiation; mice, inbred balb c; skin; wound healing; gene expression regulation; in situ hybridization; cloning, molecular; fluorescent antibody technique; amino acid sequence; growth regulation; base sequence; rats; dna primers; up-regulation; protein determination; myogenin; myoblast; muscles; muscle regeneration; skin injury; muscle proteins; muscle protein; female; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 274 |
| Issue: | 19 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1999-05-07 |
| Start Page: | 13305 |
| End Page: | 13310 |
| Language: | English |
| DOI: | 10.1074/jbc.274.19.13305 |
| PUBMED: | 10224091 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
