Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis Journal Article
| Authors: | Artero, R.; Furlong, E. E.; Beckett, K.; Scott, M. P.; Baylies, M. |
| Article Title: | Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis |
| Abstract: | Drosophila muscles originate from the fusion of two types of myoblasts, founder cells (FCs) and fusion-competent myoblasts (FCMs). To better understand muscle diversity and morphogenesis, we performed a large-scale gene expression analysis to identify genes differentially expressed in FCs and FCMs. We employed embryos derived from Toll10b mutants to obtain primarily muscle-forming mesoderm, and expressed activated forms of Ras or Notch to induce FC or FCM fate, respectively. The transcripts present in embryos of each genotype were compared by hybridization to cDNA microarrays. Among the 83 genes differentially expressed, we found genes known to be enriched in FCs or FCMs, such as heartless or hibris, previously characterized genes with unknown roles in muscle development, and predicted genes of unknown function. Our studies of newly identified genes revealed new patterns of gene expression restricted to one of the two types of myoblasts, and also striking muscle phenotypes. Whereas genes such as phyllopod play a crucial role during specification of particular muscles, others such as tartan are necessary for normal muscle morphogenesis. |
| Keywords: | signal transduction; controlled study; gene mutation; review; nonhuman; protein function; phenotype; animals; gene expression; embryo; membrane proteins; genotype; notch receptor; drosophila; embryo development; gene function; cell differentiation; cell type; embryo, nonmammalian; gene expression regulation, developmental; fluorescence in situ hybridization; oligonucleotide array sequence analysis; gene identification; receptors, notch; ras protein; dna microarray; drosophila melanogaster; ras proteins; cell adhesion; mesoderm; drosophila proteins; complementary dna; myoblast fusion; cell fusion; myoblast; receptors, cell surface; toll-like receptors; muscle, skeletal; muscle development; microarrays; myogenesis; founder cells; priority journal; toll; phyllopod; phyllopoda |
| Journal Title: | Development |
| Volume: | 130 |
| Issue: | 25 |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Date Published: | 2003-12-22 |
| Start Page: | 6257 |
| End Page: | 6272 |
| Language: | English |
| DOI: | 10.1242/dev.00843 |
| PUBMED: | 14602676 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 25 September 2014 -- Source: Scopus |
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