Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver Journal Article
| Authors: | Beckett, K.; Rochlin, K. M.; Duan, H.; Nguyen, H. T.; Baylies, M. K. |
| Article Title: | Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver |
| Abstract: | In the Drosophila embryo, body wall muscles are formed by the fusion of two cell types, Founder Cells (FCs) and Fusion Competent Myoblasts (FCMs). Using an enhancer derived from the Dmef2 gene ([C/D]*), we report the first GAL4 driver specifically expressed in FCMs. We have determined that this GAL4 driver causes expression in a subset of FCMs and, upon fusion, in developing myotubes from stage 14 onwards. In addition, we have shown that using this Dmef2-5x[C/D]*-GAL4 driver to express dominant negative Rac in only FCMs causes a partial fusion block. This novel GAL4 driver will provide a useful reagent to study Drosophila myoblast fusion and muscle differentiation. © 2007 Elsevier B.V. All rights reserved. |
| Keywords: | immunohistochemistry; controlled study; protein expression; nonhuman; protein analysis; animal cell; animals; animal tissue; embryo; drosophila; cell type; transcription factors; embryo, nonmammalian; gene expression regulation, developmental; cellular distribution; green fluorescent proteins; genes, reporter; rac protein; myocyte enhancer factor 2; transcription factor gal4; cell fusion; myoblast; myotube; fusion; muscle; myoblasts; muscle, skeletal; muscle development; genes, insect; insect development; rac; dmef2; fusion competent myoblast; gal4; lmd; animal anatomy; enhancer elements (genetics) |
| Journal Title: | Gene Expression Patterns |
| Volume: | 8 |
| Issue: | 2 |
| ISSN: | 1567-133X |
| Publisher: | Elsevier B.V. |
| Date Published: | 2008-01-01 |
| Start Page: | 87 |
| End Page: | 91 |
| Language: | English |
| DOI: | 10.1016/j.modgep.2007.10.002 |
| PUBMED: | 17988956 |
| PROVIDER: | scopus |
| PMCID: | PMC2268213 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 17 November 2011" - "CODEN: GEPEA" - "Source: Scopus" |
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