Association between molecular detection of GAGE and survival in patients with malignant melanoma: A retrospective cohort study Journal Article
| Authors: | Cheung, I. Y.; Cheung, N. K. V.; Ghossein, R. A.; Satagopan, J. M.; Bhattacharya, S.; Coit, D. G. |
| Article Title: | Association between molecular detection of GAGE and survival in patients with malignant melanoma: A retrospective cohort study |
| Abstract: | We used GAGE as a molecular marker to identify melanoma cells with metastatic potential in the peripheral blood and the bone marrow. One hundred thirty-three patients with malignant melanoma (21 clinical stage II, 74 stage III, and 38 stage IV) had a single marrow and/or blood sample drawn immediately prior to surgical resection. Simultaneous bone marrow and blood samples (85 patients), marrow-only samples (35 patients), and blood-only samples (13 patients) were examined for the presence of GAGE expression using reverse transcription. PCR. GAGE expression was associated with adverse overall patient survival, measured from the time of sampling (P = 0.01). When data were stratified for clinical stage, median survival was statistically longer among GAGE-negative patients in the stage III cohort only (P = 0.01). In a multivariate model, only GAGE positivity in blood and/or marrow and clinical stage were significant prognostic variables. It was the detection of GAGE in blood but not marrow that was associated with poor survival. The detection of blood GAGE by reverse transcription-PCR has significant adverse implications for overall survival of patients with malignant melanoma in this cohort, and it warrants further investigation. |
| Keywords: | adult; cancer survival; controlled study; aged; aged, 80 and over; middle aged; survival rate; retrospective studies; oncoprotein; major clinical study; disease marker; cancer staging; neoplasm staging; melanoma; reverse transcription polymerase chain reaction; cohort studies; bone marrow; neoplasm proteins; antigens, neoplasm; reverse transcriptase polymerase chain reaction; nucleotide sequence; predictive value of tests; melanoma cell; protein determination; biochemical marker; humans; human; male; female; priority journal; article |
| Journal Title: | Clinical Cancer Research |
| Volume: | 5 |
| Issue: | 8 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1999-08-01 |
| Start Page: | 2042 |
| End Page: | 2047 |
| Language: | English |
| PUBMED: | 10473084 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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