Prognostic significance of peripheral blood and bone marrow tyrosinase messenger RNA in malignant melanoma Journal Article


Authors: Ghossein, R. A.; Coit, D.; Brennan, M.; Zhang, Z. F.; Wang, Y.; Bhattacharya, S.; Houghton, A.; Rosai, J.
Article Title: Prognostic significance of peripheral blood and bone marrow tyrosinase messenger RNA in malignant melanoma
Abstract: The objectives of this study were to evaluate the prognostic significance of reverse transcription PCR (RT-PCR) detection of tyrosinase mRNA in the peripheral blood (PB) and bone marrow (BM) of patients with stage II-IV malignant melanoma (MM). Seventy-three PB samples and 109 BM aspirates from 123 assessable patients with stage II-IV MM were analyzed for tyrosinase mRNA using nested RT-PCR. Twenty-five controls without MM were also evaluated. The RT-PCR results were correlated with overall survival (OS) and clinical stage. Overall, 23 of the 123 patients with MM (19%) had tyrosinase mRNA in their blood and/or BM. RT-PCR positivity was present in the PB of 9 of 73 patients (12%), whereas 18 of 109 (16.5%) had tyrosinase mRNA in their BM. All controls were tyrosinase PCR negative. There was no correlation between RT-PCR results and clinical stage. Within stage II, BM PCR-positive patients had a shorter median survival (24 months) than BM PCR-negative individuals (median not reached), with a P approaching significance (P = 0.06). There was a statistically significant correlation between blood PCR positivity and decreased overall survival (P = 0.03) in all patients. Blood PCR positivity was associated with a significantly decreased OS in stage II and (P = 0.01 and 0.02, respectively) and was not a predictor of OS in stage IV. In multivariate analysis, blood RT-PCR for tyrosinase mRNA was found to be an independent predictor of survival (P = 0.03; risk ratio, 2.87). RT-PCR can specifically detect tyrosinase mRNA in the PB and BM of patients with MM. Blood RT-PCR is an independent predictor of overall survival in stage II-III MM. Additional studies are needed to define the potential role of this assay in the management of patients with advanced melanoma.
Keywords: adult; cancer survival; human tissue; aged; aged, 80 and over; middle aged; advanced cancer; cancer staging; neoplasm staging; sensitivity and specificity; polymerase chain reaction; melanoma; reverse transcription polymerase chain reaction; bone marrow; cell differentiation; transcription, genetic; tumor cells, cultured; messenger rna; blood sampling; rna, messenger; neoplasm metastasis; melanoma cell; monophenol monooxygenase; analysis of variance; tissue specificity; humans; prognosis; human; male; female; priority journal; article
Journal Title: Clinical Cancer Research
Volume: 4
Issue: 2
ISSN: 1078-0432
Publisher: American Association for Cancer Research  
Date Published: 1998-02-01
Start Page: 419
End Page: 428
Language: English
PUBMED: 9516931
PROVIDER: scopus
DOI/URL:
Notes: Source: Scopus
Citation Impact
MSK Authors
  1. Murray F Brennan
    1013 Brennan
  2. Ronald A Ghossein
    377 Ghossein
  3. Juan Rosai
    181 Rosai
  4. Daniel Coit
    501 Coit
  5. Alan N Houghton
    343 Houghton
  6. Zuo-Feng Zhang
    102 Zhang