Reverse transcriptase polymerase chain reaction (RT-PCR) detection of melanoma-related transcripts in the peripheral blood and bone marrow of patients with malignant melanoma. What have we learned? Journal Article


Authors: Ghossein, R. A.; Bhattacharya, S.; Coit, D. G.
Article Title: Reverse transcriptase polymerase chain reaction (RT-PCR) detection of melanoma-related transcripts in the peripheral blood and bone marrow of patients with malignant melanoma. What have we learned?
Abstract: The detection of circulating tumor cells (CTC) and bone marrow micrometastases (BMM) by reverse transcriptase polymerase chain reaction (RT-PCR) may help predict recurrence and survival in malignant melanoma (MM). Since the appearance of the original article by Smith et al. in 1991 (Lancet 338:1227), several groups have attempted the detection of CTC and BMM in MM using RT-PCR for melanocytic specific markers, mainly tyrosinase mRNA. Most studies show that tyrosinase is not present in the PB and BM of control individuals without MM. The PCR positivity rates in MM are extremely variable, ranging from 0% to 100%. There was a correlation between RT-PCR and stage in some but not all of the studies. These disparate findings could in part be explained by differences in RNA extraction and blood separation techniques, to unrecognized contamination leading to false positive results, or differences in patient selection. Despite these discrepancies, we and others have shown that RT-PCR for tyrosinase mRNA in PB is able to predict overall survival (OS) and disease-free survival (DFS) in a statistically significant manner. In AJCC stage II-IV patients rendered surgically free of disease, we found that blood tyrosinase positivity was an independent predictor of OS and DFS. We also found that BM tyrosinase positivity is an independent predictor of DFS in the same group of patients. RT-PCR may help identify subgroups of patients at high risk for early relapse for more aggressive adjuvant therapy. Large prospective studies and interlaboratory quality assurance initiatives are necessary to confirm the accuracy and prognostic value of these RT-PCR assays.
Keywords: genetics; review; methodology; laboratory diagnosis; melanoma; reverse transcription polymerase chain reaction; gene expression profiling; bone marrow; tumor markers, biological; genetic transcription; transcription, genetic; enzymology; pathology; tumor marker; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; monophenol monooxygenase; false positive reactions; tumor embolism; neoplasm circulating cells; humans; human
Journal Title: Recent Results in Cancer Research
Volume: 158
ISSN: 0080-0015
Publisher: Springer Verlag  
Date Published: 2001-01-01
Start Page: 63
End Page: 77
Language: English
PUBMED: 11092034
PROVIDER: scopus
DOI: 10.1007/978-3-642-59537-0_7
DOI/URL:
Notes: Chapter in "Minimal Residual Disease in Melanoma: Biology, Detection and Clinical Relevance" (ISBN: 978-3-642-64015-5) -- Export Date: 21 May 2015 -- Source: Scopus
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  1. Ronald A Ghossein
    377 Ghossein
  2. Daniel Coit
    501 Coit