GD2 synthase: A new molecular marker for detecting neuroblastoma Journal Article
| Authors: | Lo Piccolo, M. S.; Cheung, N. K. V.; Cheung, I. Y. |
| Article Title: | GD2 synthase: A new molecular marker for detecting neuroblastoma |
| Abstract: | BACKGROUND. Neuroblastomas (NBs) almost ubiquitously express the ganglioside GD2. GD2 synthesis is dependent on the key enzyme GD2 synthase. Thus, GD2 synthase transcript may prove to be a potential molecular marker of NB. METHODS. Seventy-seven NB tumor tissues of all stages, 5 NB cell lines, and 26 normal bone marrows (BMs) and peripheral blood (PBL) samples, as well as 26 non-NB remission-BMs were analyzed for the expression of GD2 synthase by a highly sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) and chemiluminescence detection. One hundred fifty-two NB BMs were tested and comparisons were made among three independent detection techniques, namely GD2 synthase RT-PCR, immunofluorescence (IF), and histology (HIST). RESULTS. GD2 synthase transcript was present in 5 of 5 cell lines and in 77 of 77 tumors tested. Among 116 marrows that were positive by at least 1 of the 3 methods, 78% were detectable by GD2 synthase, 68% by IF, and 46% by HIST. Seventy-six percent of positive BMs that were obtained during treatment and follow-up had GD2 synthase expression, whereas only 29% were HIST positive. Correlation between RT-PCR and IF was high (P = 0.001), and positivity by 3 out of 3 methods was strongly correlated with poor survival (P < 0.01). Of note, marrows tested at the time of chemotherapy were positive by at least 2 out of 3 methods and were associated with adverse outcome (P = 0.01). Serial samples (n = 28) in 5 patients demonstrated close agreement between RT-PCR and patient disease status. CONCLUSIONS. The current study found that molecular detection of GD2 synthase transcript in NB BMs may have potential value in detecting rare tumor cells. © 2001 American Cancer Society. |
| Keywords: | protein expression; survival analysis; retrospective studies; human cell; cancer diagnosis; neoplasm staging; reverse transcription polymerase chain reaction; bone marrow; tumor markers, biological; cell line; immunofluorescence; tumor cells, cultured; tumor marker; histology; rna; fluorescent antibody technique; neuroblastoma; ganglioside gd2; blood sampling; reverse transcriptase polymerase chain reaction; rt-pcr; synthetase; n-acetylgalactosaminyltransferases; chemiluminescent measurements; chemoluminescence; humans; human; priority journal; article; chemiluminescence; molecular detection |
| Journal Title: | Cancer |
| Volume: | 92 |
| Issue: | 4 |
| ISSN: | 0008-543X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2001-08-15 |
| Start Page: | 924 |
| End Page: | 931 |
| Language: | English |
| DOI: | 10.1002/1097-0142(20010815)92:4<924::aid-cncr1402>3.0.co;2-o |
| PUBMED: | 11550167 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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