Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells Journal Article
| Authors: | Zhang, F.; Altorki, N. K.; Mestre, J. R.; Subbaramaiah, K.; Dannenberg, A. J. |
| Article Title: | Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells |
| Abstract: | We investigated whether curcumin, a chemopreventive agent, inhibited chenodeoxycholate (CD)- or phorbol ester (PMA)-mediated induction of cyclooxygenase-2 (COX-2) in several gastrointestinal cell lines (SK-GT-4, SCC450, IEC-18 and HCA-7). Treatment with curcumin suppressed CD- and PMA-mediated induction of COX-2 protein and synthesis of prostaglandin E2. Curcumin also suppressed the induction of COX-2 mRNA by CD and PMA. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with CD or PMA and these effects were inhibited by curcumin. Treatment with CD or PMA increased binding of AP-1 to DNA. This effect was also blocked by curcumin. In addition to the above effects on gene expression, we found that curcumin directly inhibited the activity of COX-2. These data provide new insights into the anticancer properties of curcumin. |
| Keywords: | human cell; membrane proteins; transcription, genetic; messenger rna; rna, messenger; cyclooxygenase 2 inhibitors; cyclooxygenase 2; anti-inflammatory agents, non-steroidal; anticarcinogenic agents; prostaglandin e2; epithelium cell; isoenzymes; curcumin; phorbol ester; enzyme induction; tetradecanoylphorbol acetate; intestinal mucosa; digestive system; gastric mucosa; prostaglandin-endoperoxide synthases; bile acid; cyclooxygenase inhibitors; humans; human; priority journal; article; chenodeoxycholic acid |
| Journal Title: | Carcinogenesis |
| Volume: | 20 |
| Issue: | 3 |
| ISSN: | 0143-3334 |
| Publisher: | Oxford University Press |
| Date Published: | 1999-03-01 |
| Start Page: | 445 |
| End Page: | 451 |
| Language: | English |
| PUBMED: | 10190560 |
| PROVIDER: | scopus |
| DOI: | 10.1093/carcin/20.3.445 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
