Resveratrol inhibits the expression of cyclooxygenase-2 in human mammary and oral epithelial cells Journal Article
| Authors: | Subbaramaiah, K.; Michaluart, P.; Chung, W. J.; Dannenberg, A. J. |
| Article Title: | Resveratrol inhibits the expression of cyclooxygenase-2 in human mammary and oral epithelial cells |
| Abstract: | We determined whether resveratrol, a phenolic anti-oxidant found in grapes and wine, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induced COX-2 mRNA and protein and caused a marked increase in PGE2 synthesis. These effects were inhibited by resveratrol. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. Resveratrol inhibited PMA-mediated activation of protein kinase C (PKC) and the induction of COX-2 promoter activity by c-Jun. Phorbol ester-mediated induction of AP-1 activity was blocked by resveratrol. In addition to the above effects on COX-2 gene expression, resveratrol also directly inhibited COX-2 enzyme activity. These data are likely to be important for understanding the anti-inflammatory and anti-cancer properties of resveratrol. |
| Keywords: | controlled study; protein expression; human cell; conference paper; enzyme inhibition; gene expression; enzyme activation; messenger rna; cyclooxygenase 2; prostaglandin e2; breast epithelium; epithelium cell; protein kinase c; resveratrol; mouth mucosa; enzyme induction; pkc; phenol derivative; phorbol 13 acetate 12 myristate; prostaglandin synthesis; cox-2; human; oncogene c jun; vitaceae; pma |
| Journal Title: | Pharmaceutical Biology |
| Volume: | 36 |
| Issue: | Suppl. |
| ISSN: | 1388-0209 |
| Publisher: | Taylor & Francis Group |
| Date Published: | 1998-01-01 |
| Start Page: | 35 |
| End Page: | 43 |
| Language: | English |
| DOI: | 10.1076/phbi.36.6.35.4551 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Conference Paper -- Export Date: 12 December 2016 -- Source: Scopus |
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