Immunohistochemical evaluation of FLI-1 in acute lymphoblastic lymphoma (ALL): A potential diagnostic pitfall Journal Article
| Authors: | Lin, O.; Filippa, D. A.; Teruya-Feldstein, J. |
| Article Title: | Immunohistochemical evaluation of FLI-1 in acute lymphoblastic lymphoma (ALL): A potential diagnostic pitfall |
| Abstract: | Cases of CD45-negative acute lymphoblastic lymphoma/leukemia (ALL) immunoreactive for CD99 and Friend Leukemia Integration-1 (FLI-1) can occur and may lead to a misdiagnosis of Ewing sarcoma/peripheral neuroectodermal tumor with critical clinical treatment management implications. The objective of this study was to evaluate a panel of antibodies that would allow greater diagnostic accuracy of ALL and evaluate the frequency of FLI-1 immunoreactivity in a series of ALL cases and an expanded series of T-cell lymphoma subtypes. Immunoreactivity for CD3 was seen in 12/20 (60%), CD20 in 5/20 (25%), CD43 in 19/20(95%), CD45 in 15/20(75%), CD99 in 15/20 (75%), FLI-1, and terminal deoxynucleotidyl transferase (TdT) in 17/20 (85%) cases. Two cases negative for leukocyte common antigen (LCA), CD20, and CD3 were positive for FLI-1, CD99, TdT, and CD43. Two other LCA-negative cases were positive for CD99 but negative for FLI-1. The majority of cases showed immunoreactivity for CD43 and/or TdT. Therefore, CD43 and/or TdT should be included in the immunohistochemical evaluation of small round blue cell tumors. Absence of immunoreactivity for LCA does not exclude ALL and immunoreactivity of FLI-1 is not restricted to Ewing sarcoma/peripheral neuroectodermal tumor. We also report FLI-1 expression in an expanded series of 75 cases of T-cell lymphoma and found high expression in anaplastic large cell lymphoma and angioimmunoblastic T-cell lymphoma. © 2009 Lippincott Williams & Wilkins, Inc. |
| Keywords: | immunohistochemistry; human tissue; major clinical study; differential diagnosis; immunoreactivity; ewing sarcoma; t cell lymphoma; lymphoma; precursor cell lymphoblastic leukemia-lymphoma; large cell lymphoma; transcription factor fli 1; neuroectoderm tumor; tumor diagnosis; acute disease; acute lymphoblastic leukemia/lymphoma (all); ewing sarcoma/primitive neuroectodermal tumor (es/pnet); fli1; cd99 antigen; acute lymphoblastic lymphoma; proto-oncogene protein c-fli-1 |
| Journal Title: | Applied Immunohistochemistry & Molecular Morphology |
| Volume: | 17 |
| Issue: | 5 |
| ISSN: | 1541-2016 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2009-10-01 |
| Start Page: | 409 |
| End Page: | 412 |
| Language: | English |
| DOI: | 10.1097/PAI.0b013e3181972b6d |
| PUBMED: | 19349856 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 30 November 2010" - "CODEN: AIMMF" - "Source: Scopus" |
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