Progress in reflectance confocal microscopy for imaging oral tissues in vivo Conference Paper

  


Authors: Peterson, G.; Zanoni, D. K.; Migliacci, J.; Cordova, M.; Rajadhyaksha, M.; Patel, S.
Title: Progress in reflectance confocal microscopy for imaging oral tissues in vivo
Conference Title: Photonic Therapeutics and Diagnostics XII
Abstract: We report progress in development and feasibility testing of reflectance confocal microscopy (RCM) for imaging in the oral cavity of humans. We adapted a small rigid relay telescope (120mm long x 14mm diameter) and a small water immersion objective lens (12mm diameter, NA 0.7) to a commercial handheld RCM scanner (Vivascope 3000, Caliber ID, Rochester NY). This scanner is designed for imaging skin but we adapted the front end (the objective lens and the stepper motor that axially translates) for intra-oral use. This adaption required a new approach to address the loss of the automated stepper motor for acquisition of images in depth. A helical spring-like cap (with a coverslip to contact tissue) was designed for approximately 150 um of travel. Additionally other methods for focusing optics were designed and evaluated. The relay telescope optics is being tested in a clinical setting. With the capture of video and "video-mosaicing", extended areas can be imaged. The feasibility of imaging oral tissues was initially investigated in volunteers. RCM imaging in buccal mucosa in vivo shows nuclear and cellular detail in the epithelium and epithelial junction, and connective tissue and blood flow in the underlying lamina propria. Similar detail, including filiform and fungiform papillae, can be seen on the tongue in vivo. Clinical testing during head and neck surgery is now in progress and patients are being imaged for both normal tissue and cancerous margins in lip and tongue mucosa. © 2016 SPIE.
Keywords: scanning; head and neck surgery; reflectance confocal microscopy; confocal microscopy; histology; diagnosis; collagen; reflectance confocal microscopies; reflection; tissue; musculoskeletal system; oral mucosa; clinical settings; connective tissues; optical instrument lenses; intra oral; stepping motors; telescopes; clinical testing; feasibility testing
Journal Title Proceedings of SPIE
Volume: 9689
Conference Dates: 2016 13–14 Feb
Conference Location: San Francisco, CA
ISBN: 0277-786X
Publisher: SPIE  
Date Published: 2016-02-13
Start Page: 96891X
Language: English
DOI: 10.1117/12.2213692
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973402933&partnerID=40&md5=db83edb40e5bcad21d6606e4e2ffc4e2
Notes: Conference Paper -- Conference code: 121711 -- Export Date: 1 July 2016 -- The Society of Photo-Optical Instrumentation Engineers (SPIE) -- 13 February 2016 through 14 February 2016 -- Source: Scopus
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MSK Authors
  1. Snehal G Patel
    412 Patel
  2. Milind Rajadhyaksha
    223 Rajadhyaksha
  3. Jocelyn C Migliacci
    104 Migliacci
  4. Gary Peterson
    30 Peterson
  5. Miguel A Cordova
    88 Cordova
  6. Daniella Karassawa Zanoni
    36 Karassawa Zanoni
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