Combining wide-field imaging and reflectance confocal microscopy in a single handheld device Conference Paper
| Authors: | Smith, N. J.; Wigle, J.; Aist, J.; Kose, K.; Peterson, G.; Blough, G.; Baltzer, Z.; Hemmer, P.; Fox, W.; Rajadhyaksha, M.; Dickensheets, D. L. |
| Title: | Combining wide-field imaging and reflectance confocal microscopy in a single handheld device |
| Conference Title: | BiOS 2025: Photonics in Dermatology and Plastic Surgery 2025 |
| Abstract: | Reflectance Confocal Microscopy (RCM) imaging shows cellular-level morphology of skin in vivo, noninvasively detecting skin lesions with high sensitivity (80-95%) and specificity (70-90%) that is 2-3X higher than that of dermoscopy. The higher specificity helps reduce false positives in dermoscopically indeterminate lesions and thus reduces biopsies of benign skin lesions by 50-70%. RCM imaging is advancing into routine use at the bedside to guide patient care in clinical settings worldwide. However, a significant challenge faced by clinicians when performing RCM imaging is knowing unambiguously the location of the small RCM field-of-view (FOV) within the context of a larger lesion. The objective lens, which has a large numerical aperture (NA) and very short working distance, obscures the skin surface surrounding the location of RCM imaging. This problem can be addressed by combining a wide-field (WF) surface imaging approach with RCM imaging within a single device. We demonstrate feasibility of WF surface imaging combined with RCM imaging by integrating a micro-camera within the objective lens of a handheld confocal microscope. We report a novel objective lens design that introduces an air gap before the final hemisphere lens element, creating space for a small WF micro-camera. The micro-camera provides long depth of field and wide FOV and is integrated inside the objective lens without significantly increasing its size or decreasing optical sectioning and resolution of RCM imaging. Preliminary clinical testing on human volunteer subjects demonstrates the new handheld microscope's ability to display a WF view surrounding the location of RCM imaging on melanocytic and non-melanocytic skin lesions. © 2025 SPIE. |
| Keywords: | confocal microscopy; reflectance confocal microscopies; optical microscopy; noninvasive medical procedures; wide field imaging; photoacoustic microscopy; microscope objective; wide-field; micro-camera inside a microscope objective; reflectance confocal microscopy rcm; wide-field imaging; wide-field integration with rcm; micro cameras; reflectance confocal microscopy reflectance confocal microscopy; wide-field integration with reflectance confocal microscopy |
| Journal Title | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume: | 13292 |
| Conference Dates: | 2025 Jan 25-27 |
| Conference Location: | San Francisco, CA |
| ISBN: | 1605-7422 |
| Publisher: | SPIE |
| Date Published: | 2025-01-01 |
| Start Page: | 1329203 |
| Language: | English |
| DOI: | 10.1117/12.3040558 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | This conference paper was published in a proceedings book with ISBN: 9781510683327 -- Source: Scopus |
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82Kose
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