Wide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens Journal Article

   


Authors: Dickensheets, D. L.; Kreitinger, S.; Peterson, G.; Heger, M.; Rajadhyaksha, M.
Article Title: Wide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens
Abstract: Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera within the objective lens used for confocal microscopy, providing simultaneous sub-surface cellular imaging and WF surface morphologic imaging. The camera, inserted between a hyperhemisphere front lens and a back lens group of the objective, commands a field of view of 4.0 mm, with a resolution better than 30 μm, while confocal optical sectioning is preserved at sharper than 2.5 μm. © 2017 Optical Society of America.
Keywords: confocal microscopy; reflectance confocal microscopies; optical sectioning; field of views; cameras; lenses; objective lens; optical instrument lenses; high na objective lens; cellular imaging; separate devices; wide field imaging
Journal Title: Optics Letters
Volume: 42
Issue: 7
ISSN: 0146-9592
Publisher: Optical Society of America  
Date Published: 2017-04-01
Start Page: 1241
End Page: 1244
Language: English
DOI: 10.1364/ol.42.001241
PROVIDER: scopus
PUBMED: 28362739
PMCID: PMC5597432
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016606145&doi=10.1364%2fOL.42.001241&partnerID=40&md5=9a87fdc8cb083098a931cee2782f5186
Notes: Article -- Export Date: 2 May 2017 -- Source: Scopus
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MSK Authors
  1. Milind Rajadhyaksha
    223 Rajadhyaksha
  2. Gary Peterson
    30 Peterson
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