Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope Journal Article

   


Authors: Yin, C.; Wei, L.; Abeytunge, S.; Peterson, G.; Rajadhyaksha, M.; Liu, J. T. C.
Article Title: Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope
Abstract: There would be clinical value in a miniature optical-sectioning microscope to enable in vivo interrogation of tissues as a real-time and noninvasive alternative to gold-standard histopathology for early disease detection and surgical guidance. To address this need, a reflectance-based handheld line-scanned dual-axis confocal microscope was developed and fully packaged for label-free imaging of human skin and oral mucosa. This device can collect images at >15 frames / s with an optical-sectioning thickness and lateral resolution of 1.7 and 1.1 μm, respectively. Incorporation of a sterile lens cap design enables pressure-sensitive adjustment of the imaging depth by the user during clinical use. In vivo human images and videos are obtained to demonstrate the capabilities of this high-speed optical-sectioning microscopy device. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Keywords: microscopes; confocal microscopy; medical imaging; lateral resolution; optical sectioning; medical and biological imaging; optical microelectromechanical devices; scanning microscopy; microelectromechanical devices; label-free imaging; early disease detection; optical sectioning microscopy; pressure sensitive
Journal Title: Journal of Biomedical Optics
Volume: 24
Issue: 3
ISSN: 1083-3668
Publisher: SPIE  
Date Published: 2019-03-01
Start Page: 030501
Language: English
DOI: 10.1117/1.Jbo.24.3.030501
PROVIDER: scopus
PMCID: PMC6435977
PUBMED: 32717147
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064154033&doi=10.1117%2f1.JBO.24.3.030501&partnerID=40&md5=961e684f4a7f6ff705ea3874b5e75bbb
Notes: Article -- Export Date: 1 May 2019 -- Source: Scopus
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MSK Authors
  1. Milind Rajadhyaksha
    222 Rajadhyaksha
  2. Sanjeewa Abeytunge
    32 Abeytunge
  3. Gary Peterson
    29 Peterson
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