Identification of X-DING-CD4, a new member of human DING protein family that is secreted by HIV-1 resistant CD4(+) T cells and has anti-viral activity Journal Article


Authors: Lesner, A.; Shilpi, R.; Ivanova, A.; Gawinowicz, M. A.; Lesniak, J.; Nikolov, D.; Simm, M.
Article Title: Identification of X-DING-CD4, a new member of human DING protein family that is secreted by HIV-1 resistant CD4(+) T cells and has anti-viral activity
Abstract: We reported previously the anti-viral activity named HRF (HIV-1 Resistance Factor) secreted by HIV-1 resistant cells. This work describes the identification of HRF from cell culture supernatant of HRF-producing cells (HRF(+) cells). Employing the proteomics and cell based activity assay we recovered ten peptides sharing 80-93% sequence homology with other eukaryotic DING proteins; discrete amino acid characteristics found in our material suggested that HRF is a new member of DING proteins family and consequently we designated it as X-DING-CD4 (extracellular DING from CD4(+) T cells). The presence of X-DING-CD4 in the extracellular compartment of HRF(+) but not control HRF(-) cells was confirmed by specific anti-X-DING-CD4 antibody. Similar as the un-fractionated HRF(+) cell culture supernatant, the purified X-DING-CD4 blocked transcription of HIV-1 LTR-promoted expression of luciferase gene and replication of HIV-1 in MAGI cells. The X-DING-CD4 -mediated anti-viral activity in MAGI cells could be blocked by specific antibody. © 2009 Elsevier Inc. All rights reserved.
Keywords: unclassified drug; nonhuman; eukaryota; cd4+ t lymphocyte; cd4<sup>+</sup> t cells; ding protein; hiv; resistance; phosphate binding protein; protein ding; antiviral activity; human immunodeficiency virus 1; r factor
Journal Title: Biochemical and Biophysical Research Communications
Volume: 389
Issue: 2
ISSN: 0006-291X
Publisher: Elsevier Science, Inc.  
Date Published: 2009-11-13
Start Page: 284
End Page: 289
Language: English
DOI: 10.1016/j.bbrc.2009.08.140
PROVIDER: scopus
PMCID: PMC2957897
PUBMED: 19720052
DOI/URL:
Notes: --- - "Export Date: 30 November 2010" - "CODEN: BBRCA" - "Source: Scopus"
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MSK Authors
  1. Dimitar B Nikolov
    70 Nikolov