Identification of X-DING-CD4, a new member of human DING protein family that is secreted by HIV-1 resistant CD4(+) T cells and has anti-viral activity Journal Article
| Authors: | Lesner, A.; Shilpi, R.; Ivanova, A.; Gawinowicz, M. A.; Lesniak, J.; Nikolov, D.; Simm, M. |
| Article Title: | Identification of X-DING-CD4, a new member of human DING protein family that is secreted by HIV-1 resistant CD4(+) T cells and has anti-viral activity |
| Abstract: | We reported previously the anti-viral activity named HRF (HIV-1 Resistance Factor) secreted by HIV-1 resistant cells. This work describes the identification of HRF from cell culture supernatant of HRF-producing cells (HRF(+) cells). Employing the proteomics and cell based activity assay we recovered ten peptides sharing 80-93% sequence homology with other eukaryotic DING proteins; discrete amino acid characteristics found in our material suggested that HRF is a new member of DING proteins family and consequently we designated it as X-DING-CD4 (extracellular DING from CD4(+) T cells). The presence of X-DING-CD4 in the extracellular compartment of HRF(+) but not control HRF(-) cells was confirmed by specific anti-X-DING-CD4 antibody. Similar as the un-fractionated HRF(+) cell culture supernatant, the purified X-DING-CD4 blocked transcription of HIV-1 LTR-promoted expression of luciferase gene and replication of HIV-1 in MAGI cells. The X-DING-CD4 -mediated anti-viral activity in MAGI cells could be blocked by specific antibody. © 2009 Elsevier Inc. All rights reserved. |
| Keywords: | unclassified drug; nonhuman; eukaryota; cd4+ t lymphocyte; cd4<sup>+</sup> t cells; ding protein; hiv; resistance; phosphate binding protein; protein ding; antiviral activity; human immunodeficiency virus 1; r factor |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 389 |
| Issue: | 2 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2009-11-13 |
| Start Page: | 284 |
| End Page: | 289 |
| Language: | English |
| DOI: | 10.1016/j.bbrc.2009.08.140 |
| PROVIDER: | scopus |
| PMCID: | PMC2957897 |
| PUBMED: | 19720052 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "CODEN: BBRCA" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work