Monoubiquitinated histone H1B is required for antiviral protection in CD4+T cells resistant to HIV-1 Journal Article
| Authors: | Lesner, A.; Kartvelishvili, A.; Lesniak, J.; Nikolov, D.; Kartvelishvili, M.; Trillo-Pazos, G.; Zablotna, E.; Simm, M. |
| Article Title: | Monoubiquitinated histone H1B is required for antiviral protection in CD4+T cells resistant to HIV-1 |
| Abstract: | Linker histone H1B (H1B) coeluted with an antiviral activity during the purification of HIV-1 resistance factor (HRF) from supernatants of HRF(+) cells. Western blot analysis of the supernatant using α-H1 and α-ubiquitin antibodies detected the same band of roughly 46 kDa; this band was absent from the control supernatant. Depletion of histone from biologically active material did not affect its potential, suggesting that ubiquitinated H1B is not required for the HRF-mediated antiviral protection in HIV-1 susceptible target cells; however, specific silencing of histone H1B via RNAi in HRF(+) cells reduced the biological activity of cell culture supernatants by 96% and reversed the HIV-1 resistance phenotype of HRF(+) cells. Exposure to HRF induced ubiquitination and secretion of H1B from target HIV-1 susceptible cells, suggesting that ubiquitinated H1B is a cofactor of HRF, possibly regulating its expression and secretion from CD4+T cells induced to resist HIV-1 infection. |
| Keywords: | controlled study; protein expression; unclassified drug; human cell; nonhuman; human immunodeficiency virus infection; ubiquitin; t lymphocyte; cell protein; ubiquitination; cell culture; protein purification; nucleotide sequence; cd4-positive t-lymphocytes; western blotting; protein secretion; human immunodeficiency virus; t-lymphocyte subsets; antibodies; biochemistry; histones; hiv infections; antiviral activity; human immunodeficiency virus 1; secretion; hiv-1; cells; supernatant; purification; immunity, natural; chromatography, ion exchange; histone h1; histone h1b; hiv 1; humans; human; priority journal; article; demodulation; hiv-1 resistance factor (hrf); supernatants; hiv 1 resistance factor; virus resistance |
| Journal Title: | Biochemistry |
| Volume: | 43 |
| Issue: | 51 |
| ISSN: | 0006-2960 |
| Publisher: | American Chemical Society |
| Date Published: | 2004-11-26 |
| Start Page: | 16203 |
| End Page: | 16211 |
| Language: | English |
| DOI: | 10.1021/bi0492758 |
| PROVIDER: | scopus |
| PUBMED: | 15610014 |
| DOI/URL: | |
| Notes: | Biochemistry -- Cited By (since 1996):12 -- Export Date: 16 June 2014 -- CODEN: BICHA -- Molecular Sequence Numbers: GENBANK: NM_005322; -- Source: Scopus |
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