Definition of target antigens for naturally occurring CD4 + CD25 + regulatory T cells Journal Article
| Authors: | Nishikawa, H.; Kato, T.; Tawara, I.; Saito, K.; Ikeda, H.; Kuribayashi, K.; Allen, P. M.; Schreiber, R. D.; Sakaguchi, S.; Old, L. J.; Shiku, H. |
| Article Title: | Definition of target antigens for naturally occurring CD4 + CD25 + regulatory T cells |
| Abstract: | The antigenic targets recognized by naturally occurring CD4 + CD25 + regulatory T cells (T reg cells) have been elusive. We have serologically defined a series of broadly expressed self-antigens derived from chemically induced mouse sarcomas by serological identification of antigens by recombinant expression cloning (SEREX). CD4 + CD25 + T cells from mice immunized with SEREX-defined self-antigens had strong suppressive activity on peptide-specific proliferation of CD4 + CD25 - T cells and CD8 + T cells. The suppressive effect was observed without in vitro T cell stimulation. Foxp3 expression in these CD4 + CD25 + T cells from immunized mice was 5-10 times greater than CD4 + CD25 + T cells derived from naive mice. The suppressive effect required cellular contact and was blocked by anti-glucocorticoid-induced tumor necrosis factor receptor family-related gene antibody. In vitro suppressive activity essentially disappeared 8 wk after the last immunization. However, it was regained by in vitro restimulation with cognate self-antigen protein but not with control protein. We propose that SEREX-defined self-antigens such as those used in this study represent self-antigens that elicit naturally occurring CD4 + CD25 + T reg cells. © The Rockefeller University Press. |
| Keywords: | protein expression; dna-binding proteins; nonhuman; cd8 antigen; transcription factor foxp3; cell proliferation; t lymphocyte; forkhead transcription factors; mouse; animals; mice; cells, cultured; sarcoma; gene expression regulation; lymphocyte activation; immune tolerance; rna, messenger; nucleotide sequence; cd4-positive t-lymphocytes; autoantigens; tumor necrosis factor receptor; cd4 antigen; cell stimulation; serology; interleukin 2 receptor alpha; immunization; receptors, interleukin-2; cell contact; glucocorticoid antagonist |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 201 |
| Issue: | 5 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2005-03-07 |
| Start Page: | 681 |
| End Page: | 686 |
| Language: | English |
| DOI: | 10.1084/jem.20041959 |
| PUBMED: | 15753203 |
| PROVIDER: | scopus |
| PMCID: | PMC2212825 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 69" - "Export Date: 24 October 2012" - "CODEN: JEMEA" - "Molecular Sequence Numbers: GENBANK: AB019214, AB022159, AF055664, AF218069, D78645, U19604, U50383, X65553, XM_008348;" - "Source: Scopus" |
