Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus Journal Article
| Authors: | Price, D. L.; Li, P.; Chen, C. H.; Wong, D.; Yu, Z.; Chen, N. G.; Yu, Y. A.; Szalay, A. A.; Cappello, J.; Fong, Y.; Wong, R. J. |
| Article Title: | Silk-elastin-like protein polymer matrix for intraoperative delivery of an oncolytic vaccinia virus |
| Abstract: | Background Oncolytic viral efficacy may be limited by the penetration of the virus into tumors. This may be enhanced by intraoperative application of virus immediately after surgical resection. Methods Oncolytic vaccinia virus GLV-1h68 was delivered in silk-elastin-like protein polymer (SELP) in vitro and in vivo in anaplastic thyroid carcinoma cell line 8505c in nude mice. Results GLV-1h68 in SELP infected and lysed anaplastic thyroid cancer cells in vitro equally as effectively as in phosphate-buffered saline (PBS), and at 1 week retains a thousand fold greater infectious plaque-forming units. In surgical resection models of residual tumor, GLV-1h68 in SELP improves tumor control and shows increased viral β-galactosidase expression as compared to PBS. Conclusion The use of SELP matrix for intraoperative oncolytic viral delivery protects infectious viral particles from degradation, facilitates sustained viral delivery and transgene expression, and improves tumor control. Such optimization of methods of oncolytic viral delivery may enhance therapeutic outcomes. © 2014 Wiley Periodicals, Inc. Head Neck 38: 237-246, 2016 © 2014 Wiley Periodicals, Inc. |
| Keywords: | controlled study; protein expression; cancer surgery; unclassified drug; human cell; nonhuman; mouse; animal tissue; cell viability; green fluorescent protein; animal experiment; animal model; in vivo study; in vitro study; minimal residual disease; intraoperative period; cancer size; beta galactosidase; oncolytic virus; oncolytic virotherapy; vaccinia virus; surgery; lactate dehydrogenase; thyroid carcinoma; virus recombinant; cancer control; virus particle; polymer; drug carrier; vaccinia; viral therapy; anaplastic thyroid carcinoma; body temperature; phosphate buffered saline; human; female; priority journal; article; thyroid cancer cell line; oncolytic vaccinia virus glv 1h 68; silk elastin like protein polymer |
| Journal Title: | Head & Neck |
| Volume: | 38 |
| Issue: | 2 |
| ISSN: | 1043-3074 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 2016-02-01 |
| Start Page: | 237 |
| End Page: | 246 |
| Language: | English |
| DOI: | 10.1002/hed.23877 |
| PROVIDER: | scopus |
| PUBMED: | 25244076 |
| PMCID: | PMC4975925 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 March 2016 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work