Treatment of anaplastic thyroid carcinoma in vitro with a mutant vaccinia virus Journal Article
| Authors: | Lin, S. F.; Yu, Z.; Riedl, C.; Woo, Y.; Zhang, Q.; Yu, Y. A.; Timiryasova, T.; Chen, N.; Shah, J. P.; Szalay, A. A.; Fong, Y.; Wong, R. J. |
| Article Title: | Treatment of anaplastic thyroid carcinoma in vitro with a mutant vaccinia virus |
| Abstract: | Background: Anaplastic thyroid carcinoma (ATC) is a fatal disease resistant to all conventional treatments. Novel therapies are needed to improve dismal outcomes. Methods: A replication-competent vaccinia virus (GLV-1h68) was engineered by inserting expression cassettes encoding Renilla luciferase-green fluorescent protein (GFP), β-galactosidase, and β-glucuronidase into the genome of LIVP strain. Infection of 6 ATC cell lines by GLV-1h68 was detected in vitro at 12, 24, and 36 hours. Viral proliferation was measured through viral plaque assays. Cytotoxicity was measured daily at a multiplicity of infection (MOI) of 0.01, 0.1 and 1. Results: Viral infection was detected in all cell lines by 24 hours and increased in intensity at 36 hours. Logarithmic viral replication was detected in all cell lines. At MOI 1, <13% cell survival was measured in 5 cell lines by day 7. At MOI 0.01, 3 cell lines showed <21% cell survival by day 7. Luciferase and β-galactosidase activity at 24 hours correlated with cytotoxicity at MOI 0.01 on day 5. Conclusion: A replication-competent vaccinia virus has significant infectious and oncolytic activity against a panel of human ATC. These results encourage future in vivo and clinical studies for this novel agent to treat this fatal cancer. © 2007 Mosby, Inc. All rights reserved. |
| Keywords: | controlled study; protein expression; survival rate; unclassified drug; human cell; antineoplastic agent; cell survival; cell division; green fluorescent protein; cell line; luciferase; cytotoxicity; enzyme activity; cell line, tumor; correlation analysis; genetic engineering; carcinoma; beta galactosidase; oncolytic virotherapy; vaccinia virus; green fluorescent proteins; thyroid carcinoma; thyroid neoplasms; virus infection; glv 1h68; virus replication; indoles; luciferases; virus strain; mutagenesis; galactosides; beta-galactosidase; beta glucuronidase; virus culture |
| Journal Title: | Surgery |
| Volume: | 142 |
| Issue: | 6 |
| ISSN: | 0039-6060 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2007-12-01 |
| Start Page: | 976 |
| End Page: | 983 |
| Language: | English |
| DOI: | 10.1016/j.surg.2007.09.017 |
| PUBMED: | 18063085 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 19" - "Export Date: 17 November 2011" - "CODEN: SURGA" - "Source: Scopus" |
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