Vaccinia virus GLV-1h153 in combination with (131)I shows increased efficiency in treating triple-negative breast cancer Journal Article
| Authors: | Gholami, S.; Chen, C. H.; Lou, E.; Belin, L. J.; Fujisawa, S.; Longo, V. A.; Chen, N. G.; Gonen, M.; Zanzonico, P. B.; Szalay, A. A.; Fong, Y. M. |
| Article Title: | Vaccinia virus GLV-1h153 in combination with (131)I shows increased efficiency in treating triple-negative breast cancer |
| Abstract: | We investigated the therapeutic efficacy of a replication-competent oncolytic vaccinia virus, GLV-1h153, carrying human sodium iodide symporter (hNIS), in combination with radioiodine in an orthotopic triple-negative breast cancer (TNBC) murine model. In vitro viral infection was confirmed by immunoblotting and radioiodine uptake assays. Orthotopic xenografts (MDA-MB-231 cells) received intratumoral injection of GLV-1h153 or PBS. One week after viral injection, xenografts were randomized into 4 treatment groups: GLV-1h153 alone, GLV-1h153 and I-131 (approximate to 5 mCi), I-131 alone, or PBS, and followed for tumor growth. Kruskal-Wallis and Wilcoxon tests were performed for statistical analysis. Radiouptake assay showed a 178-fold increase of radioiodine uptake in hNIS-expressing infected cells compared with PBS control. Systemic I-131-iodide in combination with GLV-1h153 resulted in a 6-fold increase in tumor regression (24 compared to 146 mm(3) for the virus-only treatment group; P<0.05; d 40). We demonstrated that a novel vaccinia virus, GLV-1h153, expresses hNIS, increases the expression of the symporter in TNBC cells, and serves both as a gene marker for noninvasive imaging of virus and as a vehicle for targeted radionuclide therapy with I-131.Gholami, S., Chen, C-H., Lou, E., Belin, L. J., Fujisawa, S., Longo, V. A. Chen, N. G., Gonen, M., Zanzonico, P. B., Szalay, A. A., Fong, Y. Vaccinia virus GLV-1h153 in combination with I-131 shows increased efficiency in treating triple-negative breast cancer. |
| Keywords: | lung; radiation therapy; in-vitro; therapy; gene-expression; papillary; nude-mice; bone metastases; agent; sodium-iodide symporter; radioiodine; symporter; differentiated thyroid-carcinoma |
| Journal Title: | FASEB Journal |
| Volume: | 28 |
| Issue: | 2 |
| ISSN: | 0892-6638 |
| Publisher: | Federation of American Societies for Experimental Biology |
| Date Published: | 2014-02-01 |
| Start Page: | 676 |
| End Page: | 682 |
| Language: | English |
| ACCESSION: | WOS:000331072200015 |
| DOI: | 10.1096/fj.13-237222 |
| PROVIDER: | wos |
| PMCID: | PMC4005795 |
| PUBMED: | 24186964 |
| Notes: | Article -- Source: Wos |
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