Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm Journal Article

   

Authors:	Kwon, G. S.; Hadjantonakis, A. K.
Article Title:	Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm
Abstract:	Transthyretin (Ttr) is a thyroid hormone transport protein secreted by cells of the visceral yolk sac and fetal liver in developing embryos, and by hepatocytes and the choroid plexus epithelium of the brain in adult mice. Spatiotemporal localization of Ttr mRNA during embryogenesis suggested that Ttr regulatory elements might drive transgene expression throughout the visceral endoderm of early mouse embryos. We use Ttr cis-regulatory elements to generate Ttr::RFP and Ttr::Cre strains of mice, driving red fluorescent protein (RFP) and a nuclear-localized Cre recombinase, respectively. Visualization of RFP fluorescence in Ttr::RFP transgenics confirms reporter localization throughout the visceral endoderm in early embryos and in the visceral yolk sac and fetal liver of later stage embryos. Using both GFP-based and LacZ-based Cre reporter strains, we demonstrate that in Ttr::Cre transgenics, Cre-mediated recombination occurs throughout the visceral endoderm. The Ttr::Cre strain can therefore be used as a tool for genetic modifications within the visceral endoderm lineage. © 2009 Wiley-Liss, Inc.
Keywords:	controlled study; protein expression; nonhuman; protein function; pancreas; animal cell; mouse; animal tissue; mus; embryo; green fluorescent protein; enzyme activity; red fluorescent protein; transgenic animal; transgenic mouse; mus musculus; dna modification; genetic recombination; limb; beta galactosidase; cell nucleus; fetus; cre recombinase; fluorescence analysis; intestine; enzyme localization; cre reporter; fetal liver; flourescent protein; rfp; stomach; transgenic mice; transthyretin; visceral endoderm; yolk sac; cis acting element; prealbumin; endoderm; viscera
Journal Title:	Genesis
Volume:	47
Issue:	7
ISSN:	1526-954X
Publisher:	Wiley Blackwell
Date Published:	2009-07-01
Start Page:	447
End Page:	455
Language:	English
DOI:	10.1002/dvg.20522
PROVIDER:	scopus
PMCID:	PMC2878311
PUBMED:	19415627
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-70349179489&partnerID=40&md5=1a57ef757931048a9e7cf1fbd2d80a61
Notes:	--- - "Cited By (since 1996): 6" - "Export Date: 30 November 2010" - "CODEN: GNESF" - "Source: Scopus"

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