Tg(Afp-GFP) expression marks primitive and definitive endoderm lineages during mouse development Journal Article

   

Authors:	Kwon, G. S.; Fraser, S. T.; Eakin, G. S.; Mangano, M.; Isern, J.; Sahr, K. E.; Hadjantonakis, A. K.; Baron, M. H.
Article Title:	Tg(Afp-GFP) expression marks primitive and definitive endoderm lineages during mouse development
Abstract:	Alpha-fetoprotein (Afp) is the most abundant serum protein in the developing embryo. It is secreted by the visceral endoderm, its derivative yolk sac endoderm, fetal liver hepatocytes, and the developing gut epithelium. The abundance of this protein suggested that Afp gene regulatory elements might serve to effectively drive reporter gene expression in developing endodermal tissues. To this end, we generated transgenic mouse lines Tg(Afp-GFP) using an Afp promoter/enhancer to drive expression of green fluorescent protein (GFP). Bright GFP fluorescence allowed the visualization, in real time, of visceral endoderm, yolk sac endoderm, fetal liver hepatocytes, and the epithelium of the gut and pancreas. Comparison of the localization of green fluorescence with that of endogenous Afp transcripts and protein indicated that the regulatory elements used to generate these mouse lines directed transgene expression in what appeared to be all Afp-expressing cells of the embryo, but only in a subset of fetal liver cells. The bright GFP signal permitted flow cytometric analysis of fetal liver hepatocytes. These mice represent a valuable resource for live imaging as well as identification, quantitation, and isolation of cells from the primitive and definitive endoderm lineages of the developing mouse embryo. © 2006 Wiley-Liss, Inc.
Keywords:	controlled study; protein expression; promoter region; nonhuman; comparative study; flow cytometry; protein localization; pancreas; animal cell; mouse; animals; mice; animal tissue; hepatocytes; embryo; fluorescence; gfp; green fluorescent protein; embryo development; cell lineage; transgenic mouse; mus musculus; mice, transgenic; gene expression regulation, developmental; regulatory mechanism; recombinant proteins; cell isolation; transgene; reporter gene; imaging; green fluorescent proteins; pregnancy; genes, reporter; liver cell; fetal liver; transgenic mice; visceral endoderm; yolk sac; endoderm; embryo culture techniques; plasma protein; alpha-fetoprotein; alpha fetoprotein; blastocyst; fluorescent protein; enhancer region; alpha-fetoproteins; intestine epithelium; oncofetal protein; fetus liver
Journal Title:	Developmental Dynamics
Volume:	235
Issue:	9
ISSN:	1058-8388
Publisher:	Wiley Periodicals, Inc
Date Published:	2006-09-01
Start Page:	2549
End Page:	2558
Language:	English
DOI:	10.1002/dvdy.20843
PUBMED:	16708394
PROVIDER:	scopus
PMCID:	PMC1850385
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-33748306330&partnerID=40&md5=d2a84edc1cfddd72ee1428ea40768d78
Notes:	--- - "Cited By (since 1996): 25" - "Export Date: 4 June 2012" - "CODEN: DEDYE" - "Source: Scopus"

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