Lineage specificity of primary cilia in the mouse embryo Journal Article

   


Authors: Bangs, F. K.; Schrode, N.; Hadjantonakis, A. K.; Anderson, K. V.
Article Title: Lineage specificity of primary cilia in the mouse embryo
Abstract: Primary cilia are required for vertebrate cells to respond to specific intercellular signals. Here we define when and where primary cilia appear in the mouse embryo using a transgenic line that expresses ARL13B-mCherry in cilia and Centrin 2-GFP in centrosomes. Primary cilia first appear on cells of the epiblast at E6.0 and are subsequently present on all derivatives of the epiblast. In contrast, extraembryonic cells of the visceral endoderm and trophectoderm lineages have centrosomes but no cilia. Stem cell lines derived from embryonic lineages recapitulate the in vivo pattern: epiblast stem cells are ciliated, whereas trophoblast stem cells and extraembryonic endoderm (XEN) stem cells lack cilia. Basal bodies in XEN cells are mature and can form cilia when the AURKA-HDAC6 cilium disassembly pathway is inhibited. The lineage-dependent distribution of cilia is stable throughout much of gestation, defining which cells in the placenta and yolk sac are able to respond to Hedgehog ligands.
Keywords: adult; controlled study; nonhuman; animal cell; mouse; cell maturation; embryo; embryonic stem cell; embryo pattern formation; cell lineage; vertebrata; pregnancy; erinaceidae; eukaryotic flagellum; endoderm; embryo cell; aurora a kinase; centrosome; ectoderm; histone deacetylase 6; kinetosome; male; female; article
Journal Title: Nature Cell Biology
Volume: 17
Issue: 2
ISSN: 1465-7392
Publisher: Nature Publishing Group  
Date Published: 2015-02-01
Start Page: 113
End Page: 122
Language: English
DOI: 10.1038/ncb3091
PROVIDER: scopus
PUBMED: 25599390
PMCID: PMC4406239
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84923068877&partnerID=40&md5=78efd54cdf08ec652f27b15484611082
Notes: Export Date: 2 March 2015 -- Source: Scopus
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MSK Authors
  1. Kathryn Anderson
    148 Anderson
  2. Anna-Katerina Hadjantonakis
    192 Hadjantonakis
  3. Fiona Kathlen Bangs
    4 Bangs
  4. Nadine Schrode
    10 Schrode
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