Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells Journal Article

   

Authors:	Niakan, K. K.; Schrode, N.; Cho, L. T. Y.; Hadjantonakis, A. K.
Article Title:	Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells
Abstract:	At the time of implantation in the maternal uterus, the mouse blastocyst possesses an inner cell mass comprising two lineages: epiblast (Epi) and primitive endoderm (PrE). Representative stem cells derived from these two cell lineages can be expanded and maintained indefinitely in vitro as either embryonic stem (ES) or XEN cells, respectively. Here we describe protocols that can be used to establish XEN cell lines. These include the establishment of XEN cells from blastocyst-stage embryos in either standard embryonic or trophoblast stem (TS) cell culture conditions. We also describe protocols for establishing XEN cells directly from ES cells by either retinoic acid and activin-based conversion or by overexpression of the GATA transcription factor Gata6. XEN cells are a useful model of PrE cells, with which they share gene expression, differentiation potential and lineage restriction. The robust protocols for deriving XEN cells described here can be completed within 2-3 weeks. © 2013 Nature America, Inc. All rights reserved.
Keywords:	controlled study; protein expression; nonhuman; animal cell; mouse; gene expression; embryo; embryonic stem cell; cell line; cell differentiation; stem cell; animal cell culture; growth factor; retinoic acid; trophoblast; mouse embryo; blastocyst; transcription factor gata 6; extraembryonic endoderm stem cell; activin
Journal Title:	Nature Protocols
Volume:	8
Issue:	6
ISSN:	1754-2189
Publisher:	Nature Publishing Group
Date Published:	2013-06-01
Start Page:	1028
End Page:	1041
Language:	English
DOI:	10.1038/nprot.2013.049
PROVIDER:	scopus
PUBMED:	23640167
PMCID:	PMC3927835
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84879349995&partnerID=40&md5=6cff3d90f642daa772416e03cd524f62
Notes:	--- - "Export Date: 1 August 2013" - "Source: Scopus"

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