BMP4 signaling directs primitive endoderm-derived XEN cells to an extraembryonic visceral endoderm identity Journal Article

   

Authors:	Artus, J.; Douvaras, P.; Piliszek, A.; Isern, J.; Baron, M. H.; Hadjantonakis, A. K.
Article Title:	BMP4 signaling directs primitive endoderm-derived XEN cells to an extraembryonic visceral endoderm identity
Abstract:	The visceral endoderm (VE) is an epithelial tissue in the early postimplantation mouse embryo that encapsulates the pluripotent epiblast distally and the extraembryonic ectoderm proximally. In addition to facilitating nutrient exchange before the establishment of a circulation, the VE is critical for patterning the epiblast. Since VE is derived from the primitive endoderm (PrE) of the blastocyst, and PrE-derived eXtraembryonic ENdoderm (XEN) cells can be propagated in vitro, XEN cells should provide an important tool for identifying factors that direct VE differentiation. In this study, we demonstrated that BMP4 signaling induces the formation of a polarized epithelium in XEN cells. This morphological transition was reversible, and was associated with the acquisition of a molecular signature comparable to extraembryonic (ex) VE. Resembling exVE which will form the endoderm of the visceral yolk sac, BMP4-treated XEN cells regulated hematopoiesis by stimulating the expansion of primitive erythroid progenitors. We also observed that LIF exerted an antagonistic effect on BMP4-induced XEN cell differentiation, thereby impacting the extrinsic conditions used for the isolation and maintenance of XEN cells in an undifferentiated state. Taken together, our data suggest that XEN cells can be differentiated towards an exVE identity upon BMP4 stimulation and therefore represent a valuable tool for investigating PrE lineage differentiation. © 2011 Elsevier Inc.
Keywords:	hematopoiesis; visceral endoderm; live imaging; bmp; xen cell; extraembryonic endoderm; lif; afp
Journal Title:	Developmental Biology
Volume:	361
Issue:	2
ISSN:	0012-1606
Publisher:	Elsevier Inc.
Date Published:	2012-01-15
Start Page:	245
End Page:	262
Language:	English
DOI:	10.1016/j.ydbio.2011.10.015
PROVIDER:	scopus
PMCID:	PMC3246571
PUBMED:	22051107
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84355166526&partnerID=40&md5=a194ee4feb01cbe78a25386f3818f27a
Notes:	--- - "Export Date: 1 February 2012" - "CODEN: DEBIA" - "Source: Scopus"

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