Chip interacts with diverse homeodomain proteins and potentiates Bicoid activity in vivo Journal Article
| Authors: | Torigoi, E.; Bennani-Baïti, I. M; Rosen, C.; Gonzalez, K.; Morcillo, P.; Ptashne, M.; Dorsett, D. |
| Article Title: | Chip interacts with diverse homeodomain proteins and potentiates Bicoid activity in vivo |
| Abstract: | The Drosophila protein Chip potentiates activation by several enhancers and is required for embryonic segmentation. Chip and its mammalian homologs interact with and promote dimerization of nuclear LIM proteins. No known Drosophila LIM proteins, however, are required for segmentation, nor for expression of most genes known to be regulated by Chip. Here we show that Chip also interacts with diverse homeodomain proteins using residues distinct from those that interact with LIM proteins, and that Chip potentiates activity of one of these homeodomain proteins in Drosophila embryos and in yeast. These and other observations help explain the roles of Chip in segmentation and suggest a model to explain how Chip potentiates activation by diverse enhancers. |
| Keywords: | controlled study; dna-binding proteins; nonhuman; protein analysis; mammalia; animals; gene expression; models, biological; protein protein interaction; protein binding; drosophila; homeodomain proteins; transcription, genetic; nuclear proteins; transcription regulation; recombinant fusion proteins; binding site; dimerization; protein structure, tertiary; trans-activators; homeodomain protein; repressor proteins; drosophila proteins; hexapoda; chromatography, affinity; enhancer elements (genetics); promoter regions (genetics); trans-activation (genetics); priority journal; article; embryo segmentation; arachnida; insect proteins |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 97 |
| Issue: | 6 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2000-03-14 |
| Start Page: | 2686 |
| End Page: | 2691 |
| Language: | English |
| DOI: | 10.1073/pnas.050586397 |
| PUBMED: | 10688916 |
| PROVIDER: | scopus |
| PMCID: | PMC15990 |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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