The Drosophila microRNA iab-4 causes a dominant homeotic transformation of halteres to wings Journal Article
| Authors: | Ronshaugen, M.; Biemar, F.; Piel, J.; Levine, M.; Lai, E. C. |
| Article Title: | The Drosophila microRNA iab-4 causes a dominant homeotic transformation of halteres to wings |
| Abstract: | The Drosophila Bithorax Complex encodes three well-characterized homeodomain proteins that direct segment identity, as well as several noncoding RNAs of unknown function. Here, we analyze the iab-4 locus, which produces the microRNAs iab-4-5p and iab-4-3p. iab-4 is analogous to miR-196 in vertebrate Hox clusters. Previous studies demonstrate that miR-196 interacts with the Hoxb8 3′ untranslated region. Evidence is presented that miR-iab-4-5p directly inhibits Ubx activity in vivo. Ectopic expression of mir-iab-4-5p attenuates endogenous Ubx protein accumulation and induces a classical homeotic mutant phenotype: the transformation of halteres into wings. These findings provide the first evidence for a noncoding homeotic gene and raise the possibility that other such genes occur within the Bithorax complex. We also discuss the regulation of mir-iab-4 expression during development. © 2005 by Cold Spring Harbor Laboratory Press. |
| Keywords: | gene cluster; gene mutation; mutation; nonhuman; genetic analysis; phenotype; animals; microrna; gene expression; gene locus; drosophila; in vivo study; homeodomain proteins; transcription factors; vertebrata; gene expression regulation, developmental; gene interaction; 3' untranslated region; micrornas; drosophila proteins; multigene family; 3' untranslated regions; forelimb; wing; genes, insect; homeobox; homeotic gene; iab-4; ultrabithorax |
| Journal Title: | Genes and Development |
| Volume: | 19 |
| Issue: | 24 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2005-12-15 |
| Start Page: | 2947 |
| End Page: | 2952 |
| Language: | English |
| DOI: | 10.1101/gad.1372505 |
| PUBMED: | 16357215 |
| PROVIDER: | scopus |
| PMCID: | PMC1315399 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 73" - "Export Date: 24 October 2012" - "CODEN: GEDEE" - "Source: Scopus" |
