Homeotic function of drosophila bithorax-complex miRNAs mediates fertility by restricting multiple hox genes and TALE cofactors in the CNS Journal Article
| Authors: | Garaulet, D. L.; Castellanos, M. C.; Bejarano, F.; Sanfilippo, P.; Tyler, D. M.; Allan, D. W.; Sanchez-Herrero, E.; Lai, E. C. |
| Article Title: | Homeotic function of drosophila bithorax-complex miRNAs mediates fertility by restricting multiple hox genes and TALE cofactors in the CNS |
| Abstract: | The Drosophila Bithorax complex (BX-C) Hox cluster contains a bidirectionally transcribed miRNA locus, and a deletion mutant (Delta mir) lays no eggs and is completely sterile. We show these miRNAs are expressed and active in distinct spatial registers along the anterior-posterior axis in the CNS. Delta mir larvae derepress a network of direct homeobox gene targets in the posterior ventral nerve cord (VNC), including BX-C genes and their TALE cofactors. These are phenotypically critical targets, because sterility of Delta mir mutants was substantially rescued by heterozygosity of these genes. The posterior VNC contains IIp7+ oviduct motoneurons, whose innervation and morphology are defective in Delta mir females, and substantially rescued by heterozygosity of Delta mir targets, especially within the BX-C. Collectively, we reveal (1) critical roles for Hox miRNAs that determine segment-specific expression of homeotic genes, which are not masked by transcriptional regulation; and (2) that BX-C miRNAs are essential for neural patterning and reproductive behavior. |
| Keywords: | microrna; evolution; competition; expression; specificity; regulatory rnas; phenotypic suppression; extradenticle; homothorax; underlies |
| Journal Title: | Developmental Cell |
| Volume: | 29 |
| Issue: | 6 |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Date Published: | 2014-06-23 |
| Start Page: | 635 |
| End Page: | 648 |
| Language: | English |
| ACCESSION: | WOS:000338174600003 |
| DOI: | 10.1016/j.devcel.2014.04.023 |
| PROVIDER: | wos |
| PMCID: | PMC4111139 |
| PUBMED: | 24909902 |
| Notes: | Article -- Source: Wos |
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