The mir-279/996 cluster represses receptor tyrosine kinase signaling to determine cell fates in the Drosophila eye Journal Article
| Authors: | Duan, H.; de Navas, L. F.; Hu, F.; Sun, K.; Mavromatakis, Y. E.; Viets, K.; Zhou, C.; Kavaler, J.; Johnston, R. J.; Tomlinson, A.; Lai, E. C. |
| Article Title: | The mir-279/996 cluster represses receptor tyrosine kinase signaling to determine cell fates in the Drosophila eye |
| Abstract: | Photoreceptors in the crystalline Drosophila eye are recruited by receptor tyrosine kinase (RTK)/Ras signaling mediated by Epidermal growth factor receptor (EGFR) and the Sevenless (Sev) receptor. Analyses of an allelic deletion series of the mir-279/996 locus, along with a panel of modified genomic rescue transgenes, show that Drosophila eye patterning depends on both miRNAs. Transcriptional reporter and activity sensor transgenes reveal expression and function of miR-279/996 in non-neural cells of the developing eye. Moreover, mir-279/996 mutants exhibit substantial numbers of ectopic photoreceptors, particularly of R7, and cone cell loss. These miRNAs restrict RTK signaling in the eye, since mir-279/996 nulls are dominantly suppressed by positive components of the EGFR pathway and enhanced by heterozygosity for an EGFR repressor. miR-279/996 limit photoreceptor recruitment by targeting multiple positive RTK/Ras signaling components that promote photoreceptor/R7 specification. Strikingly, deletion of mir-279/996 sufficiently derepresses RTK/Ras signaling so as to rescue a population of R7 cells in R7-specific RTK null mutants boss and sev, which otherwise completely lack this cell fate. Altogether, we reveal a rare setting of developmental cell specification that involves substantial miRNA control. © 2018. Published by The Company of Biologists Ltd. |
| Keywords: | microrna; drosophila; r7 photoreceptor; rtk signaling |
| Journal Title: | Development |
| Volume: | 145 |
| Issue: | 7 |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Date Published: | 2018-04-01 |
| Start Page: | dev159053 |
| Language: | English |
| DOI: | 10.1242/dev.159053 |
| PROVIDER: | scopus |
| PUBMED: | 29540498 |
| PMCID: | PMC5963866 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 May 2018 -- Source: Scopus |
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