Functional architecture of an intracellular membrane t-SNARE Journal Article

Authors: Fukuda, R.; Mcnew, J. A.; Weber, T.; Parlati, F.; Engel, T.; Nickel, W.; Rothman, J. E.; Söllner, T. H.
Article Title: Functional architecture of an intracellular membrane t-SNARE
Abstract: Lipid bilayer fusion is mediated by SNAREs (soluble N-ethyl-maleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, of which one is supplied by the v-SNARE and the other three by the t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and a SNAP-25 protein contributes the other two. Although there are numerous homologues of syntaxin on intracellular membranes, there are only two SNAP-25-related proteins in yeast, Sec9 and Spo20, both of which are localized to the plasma membrane and function in secretion and sporulation, respectively. What replaces SNAP-25 in t-SNAREs of intracellular membranes? Here we show that an intracellular t-SNARE is built from a 'heavy chain' homologous to syntaxin and two separate non-syntaxin 'light chains'. SNAP-25 may thus be the exception rather than the rule, having been derived from genes that encoded separate light chains that fused during evolution to produce a single gene encoding one protein with two helices.
Keywords: nonhuman; protein conformation; protein analysis; protein protein interaction; nerve tissue proteins; protein binding; membrane proteins; recombinant fusion proteins; saccharomyces cerevisiae; escherichia coli; cell membrane; saccharomyces cerevisiae proteins; protein structure; protein structure, quaternary; fungal proteins; vesicular transport proteins; intracellular membranes; membrane structure; snare protein; qa-snare proteins; r-snare proteins; snare proteins; synaptosomal-associated protein 25; priority journal; article
Journal Title: Nature
Volume: 407
Issue: 6801
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2000-09-14
Start Page: 198
End Page: 202
Language: English
DOI: 10.1038/35025084
PUBMED: 11001059
PROVIDER: scopus
Notes: Export Date: 18 November 2015 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Thomas H Sollner
    65 Sollner
  2. James E Rothman
    102 Rothman
  3. Thomas Weber
    14 Weber
  4. James A Mcnew
    21 McNew
  5. Francesco Parlati
    17 Parlati
  6. Thomas A Engel
    4 Engel
  7. Ryoichi   Fukuda
    4 Fukuda
  8. Hans-Walter Karl Nickel
    8 Nickel