Binding of the synaptic vesicle v-SNARE, synaptotagmin, to the plasma membrane t-SNARE, SNAP-25, can explain docked vesicles at neurotoxin-treated synapses Journal Article

   

Authors:	Schiavo, G.; Stenbeck, G.; Rothman, J. E.; Söllner, T. H.
Article Title:	Binding of the synaptic vesicle v-SNARE, synaptotagmin, to the plasma membrane t-SNARE, SNAP-25, can explain docked vesicles at neurotoxin-treated synapses
Abstract:	Neurotransmitter release requires the specific docking of synaptic vesicles to the presynaptic plasma membrane followed by a calcium-triggered fusion event. Herein we report a previously unsuspected interaction of the synaptic vesicle protein and likely calcium sensor synaptotagmin with the plasma membrane t-SNARE SNAP-25. This interaction appears to resolve the apparent paradox that synaptic vesicles are capable of docking even when VAMP (vesicle-associated membrane protein) or syntaxin is cleaved or deleted and suggests that two species of v-SNAREs (VAMP and synaptotagmin) and two species of t-SNAREs (SNAP-25 and syntaxin) interact to functionally dock synaptic vesicles.
Keywords:	animals; nerve tissue proteins; membrane proteins; calcium; recombinant fusion proteins; membrane glycoproteins; presynaptic terminals; cell membrane; cattle; calcium-binding proteins; cerebral cortex; synaptotagmin; membrane binding; exocytosis; fusion; vesicular transport proteins; calcium transport; neurotransmission; synaptic transmission; membrane fusion; botulinum toxins; neurotransmitter release; syntaxin; docking protein; neurotransmitter; qa-snare proteins; r-snare proteins; snare proteins; synaptic vesicles; synapse vesicle; synaptosomal-associated protein 25; synaptobrevin; priority journal; article; synaptotagmins; neurotoxin
Journal Title:	Proceedings of the National Academy of Sciences of the United States of America
Volume:	94
Issue:	3
ISSN:	0027-8424
Publisher:	National Academy of Sciences
Date Published:	1997-02-04
Start Page:	997
End Page:	1001
Language:	English
DOI:	10.1073/pnas.94.3.997
PUBMED:	9023371
PROVIDER:	scopus
PMCID:	PMC19628
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031019926&doi=10.1073%2fpnas.94.3.997&partnerID=40&md5=121ac15598e23f662b6c82915f57016e
Notes:	Article -- Export Date: 17 March 2017 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/108178