Calcium-independent stimulation of membrane fusion and SNAREpin formation by synaptotagmin I Journal Article
| Authors: | Mahal, L.; Sequeira, S. M.; Gureasko, J.; Söllner, T. H. |
| Article Title: | Calcium-independent stimulation of membrane fusion and SNAREpin formation by synaptotagmin I |
| Abstract: | Neurotransmitter release requires the direct coupling of the calcium sensor with the machinery for membrane fusion. SNARE proteins comprise the minimal fusion machinery, and synaptotagmin I, a synaptic vesicle protein, is the primary candidate for the main neuronal calcium sensor. To test the effect of synaptotagmin I on membrane fusion, we incorporated it into a SNARE-mediated liposome fusion assay. Synaptotagmin I dramatically stimulated membrane fusion by facilitating SNAREpin zippering. This stimulatory effect was topologically restricted to v-SNARE vesicles (containing VAMP 2) and only occurred in trans to t-SNARE vesicles (containing syntaxin 1A and SNAP-25). Interestingly, calcium did not affect the overall fusion reaction. These results indicate that synaptotagmin I can directly accelerate SNARE-mediated membrane fusion and raise the possibility that additional components might be required to ensure tight calcium coupling. |
| Keywords: | metabolism; nerve tissue proteins; membrane proteins; calcium; physiology; membrane glycoproteins; membrane protein; calcium binding protein; calcium-binding proteins; nerve protein; liposome; liposomes; synaptotagmin; vesicular transport proteins; synaptotagmin i; vesicular transport protein; synaptic transmission; membrane fusion; snare protein; snare proteins; article; synaptotagmins |
| Journal Title: | Journal of Cell Biology |
| Volume: | 158 |
| Issue: | 2 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2002-07-22 |
| Start Page: | 273 |
| End Page: | 282 |
| Language: | English |
| PUBMED: | 12119360 |
| PROVIDER: | scopus |
| PMCID: | PMC2173117 |
| DOI: | 10.1083/jcb.200203135 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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