A t-SNARE of the endocytic pathway must be activated for fusion Journal Article
| Authors: | Paumet, F.; Brügger, B.; Parlati, F.; Mcnew, J. A.; Söllner, T. H.; Rothman, J. E. |
| Article Title: | A t-SNARE of the endocytic pathway must be activated for fusion |
| Abstract: | The t-SNARE in a late Golgi compartment (Tlg2p) syntaxin is required for endocytosis and localization of cycling proteins to the late Golgi compartment in yeast. We show here that Tlg2p assembles with two light chains, Tlg1p and Vti1p, to form a functional t-SNARE that mediates fusion, specifically with the v-SNAREs Snc1p and Snc2p. In vitro, this t-SNARE is inert, locked in a nonfunctional state, unless it is activated for fusion. Activation can be mediated by a peptide derived from the v-SNARE, which likely bypasses additional regulatory proteins in the cell. Locking t-SNAREs creates the potential for spatial and temporal regulation of fusion by signaling processes that unleash their fusion capacity. |
| Keywords: | nonhuman; protein localization; membrane proteins; amino acid sequence; molecular sequence data; saccharomyces cerevisiae; carrier proteins; protein transport; saccharomyces cerevisiae proteins; endocytosis; polyacrylamide gel electrophoresis; regulator protein; fusion; endosome; golgi complex; fungal proteins; vesicular transport proteins; membrane transport proteins; membrane fusion; snare protein; qa-snare proteins; qb-snare proteins; golgi apparatus; r-snare proteins; snare proteins; snare; priority journal; article; snc2p; tlg2p |
| Journal Title: | Journal of Cell Biology |
| Volume: | 155 |
| Issue: | 6 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2001-12-10 |
| Start Page: | 961 |
| End Page: | 968 |
| Language: | English |
| DOI: | 10.1083/jcb.200104092 |
| PUBMED: | 11739407 |
| PROVIDER: | scopus |
| PMCID: | PMC2150898 |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work